七叶内酯对平滑肌源性泡沫细胞胆固醇外转运蛋白ABCA1和ABCG1表达的影响  

Effect of the esculetin on the cholesterol transport protein ABCA1 and ABCG1 in smooth muscle-derived foam cells

作　　者：贺超[1] 黄烨[1] 李柏生[2] 周俊立[3] 胡霖 郭小梅[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院心电生理实验室,武汉430030 [2]广东省疾病预防控制中心微生物检验所,广州510300 [3]广东药学院公共卫生学院微生物教研室,广州510310 [4]湖北省武汉市第五人民医院放射科,430050

出　　处：《广东医学》2012年第22期3368-3371,共4页Guangdong Medical Journal

基　　金：国家自然科学基金面上项目(编号:30971244)

摘　　要：目的研究七叶内酯在体外干预泡沫细胞形成的分子机制,及其对胆固醇外转运蛋白三磷酸腺苷结合盒转运体A1(ABCA1)和G1(ABCG1)表达的影响。方法以平滑肌源性泡沫细胞为研究对象,不同浓度(5、10、20μg/mL)的七叶内酯体外干预平滑肌源性泡沫细胞,油红O染色观察细胞内脂滴的形成,Real-time PCR检测ABCA1和ABCG1转录水平的表达,Western blot检测泡沫细胞膜蛋白ABCA1和ABCG1和核蛋白过氧化物酶体增殖物激活受体γ(PPARγ)的磷酸化水平。结果 50μg/mL七叶内酯能通过激活PPARγ,促进胆固醇外转运蛋白ABCA1和ABCG1表达。结论七叶内酯可以激活PPARγ,促进ABCA1和ABCG1表达,抑制平滑肌源性泡沫细胞的形成。Objective To study the mechanism of esculetin on the formation of foam cells in vitro and on the expression of cholesterol transport protein ABCAI and ABCG1. Methods Different concentrations of esculetin were applied for interference with the smooth muscle - derived foam cells in vitro, while foam cell formation was detected by oil red O staining. Real - time PCR was applied for assessment of ABCA1 and ABCG1 expression; as Western - blot for the pho phorylation of membrane proteins ABCA1 and ABCG1, and nucleoprotein PPAR -γ. Results The cholesterol transport protein ABCA1 and ABCG1 were up - regulated with esculetin through the activation of PPAR - γ Conclusion Esculetin can activate PPAR - γand promote ABCA1 and ABCG1 expression, thus inhibits the formation of smooth muscle - de- rived foam cells.

关 键 词：泡沫细胞 过氧化物酶体增殖物激活受体Γ 三磷酸腺苷结合盒转运体A1 三磷酸腺苷结合盒转运体G1 

分 类 号：R543.502[医药卫生—心血管疾病]