枯草芽孢杆菌FB123所产抗菌物P3c的分离纯化及其表面活性功能分析  

Purification of Antimicrobial Substance P3c Produced by Bacillus subtilis FB123 and Analysis of Its Biosurfactant Function

作　　者：施碧红[1] 蔡良[1] 郑永标[1] 商伟伟[1] 许亦峰[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108

出　　处：《福建师范大学学报（自然科学版）》2012年第6期106-111,共6页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省自然科学基金资助项目(2009J01128);福建省科技厅重点资助项目(2009N0033)

摘　　要：枯草芽孢杆菌(Bacillus subtilis)FB123的发酵产物经酸沉淀、凝胶层析、反相层析、薄层层析获得一单一条带P3c抗菌组分,原位酸水解茚三酮显色反应显示该组分为环脂肽物质.对P3c组分抑菌及排油圈实验表明,该组分是一种具有广谱抑菌活性的生物表面活性剂.One active component of P3c was purified from the fermentation broth of Bacillus subtilis FB123 after steps of acid precipitation, gel chromatography, reversed phase chromatography and thin layer chromatography. The P3c was primary identified as a cyclic lipopeptide by the way of acid hydrolysis ninhydrine. The antimicrobial test and the oil ex- traction test showed that the P3c is a kind of biological surfactant and exhibited broad spec- trum antimicrobial activity.

关 键 词：枯草芽孢杆菌FB123 反相层析 抑菌谱 生物表面活性剂 

分 类 号：Q936[生物学—微生物学]