3种挥发油对马钱子总碱透皮贴片体外经皮渗透的影响  

Effects of three kinds of volatile oils on in vitro percutaneous penetration of total alkaloids in Strychni Semen transdermal patches

作　　者：张贵华[1] 马云淑[1] 黄金娥[1] 罗红梅[1] 康绍建 

机构地区：[1]云南中医学院,云南昆明650500 [2]云南省食品药品检验所,云南昆明650000

出　　处：《中草药》2012年第11期2158-2163,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30960517);云南省科技计划项目(2008CD152)

摘　　要：目的研究砂仁、草果、白豆蔻挥发油对马钱子总碱贴片中马钱子总碱(主要含马钱子碱和士的宁)体外经皮渗透的影响。方法以小鼠离体皮肤为实验材料,采用改良Franz扩散池法,以HPLC法测定马钱子总碱贴片中马钱子总碱(马钱子碱和士的宁)的累积渗透量(Qn),考察3%、5%、7%、10%砂仁、草果、白豆蔻挥发油对马钱子总碱(马钱子碱和士的宁)的促渗效果。结果不同质量浓度挥发油中,以5%砂仁挥发油、3%草果挥发油、10%白豆蔻挥发油渗透速率(JSS)最大,分别为1.039、0.951、0.907 mg/(cm2.h);与不加促渗剂的阴性对照组相比,增渗倍数(ER)分别为1.212、1.230、1.383,其中5%砂仁油的ER大于阳性对照10%氮酮。结论 5%砂仁油、3%草果油、10%白豆蔻油均能明显促进马钱子总碱贴片中马钱子总碱(马钱子碱和士的宁)的透皮吸收。Objective To study the effects of volatile oils in Amomi Fructus （AF）, Tsaoko Fructus （TF）, and Amomi Rotundus Fructus （ARF） on in vitro percutaneous penetration of brucine and stychnine in the total alkaloids of Strychni Semen （TASS） transdermal patches. Methods Improved Franz-cells were used for the transdermal diffusion test, and the cumulative permeation quantity （Qn） of brucine and strychnine in the TASS transdermal patches was determined by HPLC. The enhancing effects of 3%, 5%, 7%, and 10% volatile oils in AF, TF, and ARF on percutaneous diffusion of brucine and stychnine in the TASS were investigated. Results The penetration rates （Jss） of 5% volatile oil in AF, 3% volatile oil in TF, and 10% volatile oil in ARF were 1.039, 0.951, and 0.907 mg/（cm2·h）, respectively, which were the largest among the different concentration of volatile oils. Compared with the negative control group without penetrant, the enhancement ratios （ER） were 1.212, 1.230, and 1.383, respectively, and the ER of 5% volatile oil in AF was higher than that of the positive control 10% Azone. Conclusion Results show that 5% volatile oil of AF, 3% volatile oil of TF, and 10% volatile oil of ARF could obviously enhance the transdermal absorption of brucine and strychnine in the TASS transdermal patches.

关 键 词：马钱子总碱 马钱子碱 士的宁 砂仁 草果 白豆蔻 挥发油 

分 类 号：R283.6[医药卫生—中药学] R286.02[医药卫生—中医学]