长鞭红景天细胞悬浮培养体系优化研究  

Optimization of cell suspension culture system of Rhodiola fastigiata

作　　者：王莉[1] 管江红[2] 史玲玲[3] 刘玉军[3] 

机构地区：[1]西藏民族学院医学院,陕西咸阳712082 [2]西藏民族学院信息工程学院,陕西咸阳712082 [3]北京林业大学生物科学与技术学院,北京100083

出　　处：《中草药》2012年第11期2272-2278,共7页Chinese Traditional and Herbal Drugs

基　　金：教育部重点项目"长鞭红景天悬浮细胞体系对高原典型环境因子应答基因的筛选"(211176);西藏自治区科技厅项目"长鞭红景天悬浮细胞体系的抗逆性研究"

摘　　要：目的优化长鞭红景天Rhodiola fastigiata细胞悬浮培养体系及生长条件。方法研究基本培养基、激素、碳源、氮源等因素对长鞭红景天细胞生长和红景天苷产量的影响,建立高产红景天苷的细胞悬浮培养体系。结果 MS培养基+BA5.0 mg/L+2,4-D 0.1 mg/L+蔗糖30 g/L为长鞭红景天细胞悬浮培养的最适培养条件。结论对培养条件进行优化,为利用长鞭红景天细胞大规模生产天然活性成分奠定基础,为缓解野生长鞭红景天资源的匮乏提供可行途径。Objective To optimize the cell suspension culture system and growth conditions of Rhodiolafastigiata. Methods The effects of basic medium and hormone as well as its Concentration, carbon, and nitrogen sources on the growth of R. fastigiata and the yield of salidroside were investigated and the cell suspension culture system of R.fastigiata with high yield was established. Results MS ＋ BA 5.0 mg/L ＋ 2, 4-D 0. l mg/L ＋ sucrose 30 g/L was the optimal medium for obtaining cell suspension culture ofR. fastigiata with high yield. Conclusion Optimization of the culture conditions could lay a good foundation for producing natural active components through a large-scale production of R. fastigiata cells and provide a feasible way for alleviating the shortage of its natural resources.

关 键 词：长鞭红景天 细胞悬浮培养 红景天苷 培养基 激素 碳源 氮源 

分 类 号：R282.21[医药卫生—中药学]