尿1-羟基芘与外周血细胞色素P450酶关系初步研究  

Relationship between urinary 1-hydroxrpyrene level and cytochrome P450 enzyme family in peripheral blood

作　　者：王芳[1] 陈靖晗[1] 侍洪斌[2] 吴丽娜[1] 吕严[1] 

机构地区：[1]天津医科大学公共卫生学院劳动卫生与环境卫生系,天津300070 [2]天津医科大学总医院

出　　处：《环境与健康杂志》2012年第11期1032-1034,共3页Journal of Environment and Health

摘　　要：目的研究非职业多环芳烃暴露者尿中1-羟基芘水平与外周血细胞色素P450酶含量间的关系。方法于2011年12月选取50名到天津市某医院健康管理中心体检的人员,使用碱水解-高效液相色谱法测定尿1-羟基芘浓度;以双抗体夹心法测定外周血细胞色素P450酶活力;以问卷调查形式收集个人的一般情况、生活、职业史等信息。结果调整了年龄、性别和饮酒状况后,尿1-羟基芘浓度与外周血细胞色素P450酶活力存在正相关(r=0.431,P=0.001),另外电磁辐射诱导外周血细胞色素P450酶活力,食用大蒜抑制其活力。结论非职业多环芳烃暴露者尿1-羟基芘浓度与外周血细胞色素P450酶活力存在正相关,为外周血细胞色素P450酶系作为PAHs暴露早期生物标志物的可行性提供依据。Objective To investigate the relationship between the urinary 1-hydroxypyrene level and eytochrome P450 enzyme family in peripheral blood among non-occupational polycyelic aromatic hydrocarbons exposure individuals in Tianjin. Methods Fifty people undergone physical examinations to the hospital were selected. Urinary levels of 1-hydroxypyrene were measured by alkaline hydrolysis combined with high performance liquid chromatography, and cytochrome P450 enzyme family were measured with ELISA method. Results The direct correlation was observed between the urinary level of l-hydroxypyrene and cytoehrome P450 enzyme family in peripheral blood in the study subjects（r=0.431, P=0.001 ） after adjusting sex, age and alcohol drinking.The activity of cytochrome P450 enzyme could be induced by exposure to electromagnetic radiation, inhibited by intake of garlic. Conclusion Urinary levels of 1-hydroxypyrene of non-occupational polycyclic aromatic hydroearbons exposure individuals positively correlated with the aeivity of cytochrome P450 enzyme in peripheral blood.

关 键 词：多环芳烃 1-羟基芘 细胞色素P450酶 生物标志物 

分 类 号：R181.3[医药卫生—流行病学]