雏鸭感染鹅源H5N1亚型禽流感病毒后免疫器官淋巴细胞增殖功能变化  

Changes of Lymphocytes Proliferation Function in Immune Organs of Ducks Infected with H5N1 Subtype Avian Influenza Virus of Geese Origin

作　　者：于学武[1,2] 郑世民[1] 刘明[3] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]辽宁省动物疫病预防控制中心,辽宁沈阳110164 [3]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国家禽》2012年第22期12-15,共4页China Poultry

基　　金：国家自然科学基金(30371066);黑龙江省自然科学基金(C200841)

摘　　要：以21日龄雏鸭为研究对象,应用细胞培养技术和四甲基偶氮唑盐(MTT)检测法,对鹅源H5N1亚型禽流感病毒(AIV)感染雏鸭胸腺、法氏囊和脾脏的T或/和B淋巴细胞增殖功能变化进行了较全面系统的动态检测。结果发现,鹅源H5N1亚型中强毒AIV感染雏鸭后,其上述免疫器官T或/和B淋巴细胞对ConA或LPS的增殖反应均不同程度地低于相应对照雏鸭,表明AIV感染雏鸭后,其免疫器官的细胞免疫和体液免疫功能明显受抑制,上述免疫病理改变可能是导致感染雏鸭免疫抑制的主要原因之一。The dynamic changes of T and B lymphocytes proliferation function to ConA or LPS in thymus,bursa and spleen of 21-day-old ducks infected with H5N1 subtype avian influenza virus （AIV） geese origin were detected by using cellular cuhural technology and 3- [4, 5-Dimethylthiazol-2-yl J-2,5-diphenyltetrazolium bromide（MTT）method. The study revealed that the proliferation fimction of T and B lymphocytes from immune organs of ducks infected with AIV was decreased at different degrees compared with that of control group. The results showed that the cellular and humoral immune fimction in immune organs of ducks infected with AIV were suppressed.

关 键 词：雏鸭 鹅源H5N1亚型禽流感病毒 免疫器官 淋巴细胞增殖功能 

分 类 号：S855.3[农业科学—临床兽医学]