地方特色蛋鸡母本群禽白血病病毒感染的鉴定  

Identification of Avian Leucosis Virus Infection in Femal Parent Groups of Local Laying Hens

作　　者：窦新红[1,2] 秦爱建[1] 沈海玉[2] 窦套存[2] 肖芹[2] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]中国农业科学院家禽研究所,江苏扬州225125

出　　处：《中国家禽》2012年第22期16-19,共4页China Poultry

基　　金：国家“十二五”科技支撑计划(2012BAD39B04);江苏省农业三项工程项目(sx(2011)192);扬州市科技计划项目(yz2010049)

摘　　要：研究采用ELISA、病毒分离和PCR方法对地方特色蛋鸡配套系母本鸡群的ALV抗体、抗原和核酸等进行了检测,结果显示受检种鸡群泄殖腔棉拭p27抗原阳性率为47.8%,A/B抗体阳性率为4.3%,未检测到J亚群抗体阳性个体,表明鸡群感染ALV-A/B,同时分离出两株外源性禽白血病病毒并命名为JS12JD01和JS12JD02。PCR扩增其囊膜蛋白基因并测序分析,结果显示JS12JD01株与A亚群参考株的gp85氨基酸序列同源性较高,与B、C、D、E和J亚群参考株同源性仅为32.5%～82.8%;JS12JD02分离株与B亚群参考株的gp85氨基酸序列同源性较高,而与A、C、D、E和J亚群参考株的同源性仅为34.6%～88.7%。表明这两株分离株分属于A、B两个不同亚群,该鸡群存在A、B亚群ALV的混合感染。Avian leucosis virus （ALV）infection was investigated with ELISA test,DF1 cell culture and PCR in local female parent flock. The result of ELISA test showed that positive rate of＇ p27 antigen was 47.8%,positive rate of antibody against subgroup A/B was 4.3%,but there weren＇t any antibody against subgroup J positive sample. Two ALV named as JS12JDO1 and JS12JD02 were isolated via DF1 ceil culture,then by PCR amplification of the env gene. Comparison of gp85 amino acid sequences between isolation strains and other ALV strains from different subgroups data in GenBank indicated that JS12JDO1 had the higher homology with reference strains of subgroup A （88.7% to 99.1%）,but the homology with other subgroups was only between 32.5% to 82.8%. JS12JD02 had higher homology with reference strains of subgroup B （92.4% to 96,8%）,but the homology with other subgroups was only between 34.6% to 88.7%. The results indicated that there was eoninfection of ALV-A and ALV-B in local flocks,suggesting the complex trend of the epidemic of ALV in local chickens in China.

关 键 词：禽白血病 gp85 地方品系 病毒分离 

分 类 号：S852.65[农业科学—基础兽医学]