大孔树脂分离纯化荷叶中的黄酮与生物碱  

Isolation and purification of flavonoids and alkaloids from the leaf of Nelumbo nucifera Gaertn by macroporous adsorption resin

作　　者：刘东亚[1] 王谊[1] 蒋建勤[1] 

机构地区：[1]中国药科大学天然药物化学研究室,南京211198

出　　处：《西北药学杂志》2012年第6期511-514,共4页Northwest Pharmaceutical Journal

基　　金：江苏省普通高校研究生科研创新计划项目(编号:CXZZ11_0804)

摘　　要：目的研究确定荷叶的最佳分离纯化工艺。方法采用不同的树脂柱进行吸附,用分光光度法测定黄酮与生物碱的含量,用HPLC验证荷叶碱的含量,对工艺进行评价。结果 D-101型树脂较适合荷叶中的生物碱与黄酮的分离与纯化,在确定的工艺下,荷叶碱转移得率达到57.2%。结论该工艺适合荷叶中的黄酮与生物碱的分离与富集。Objective To investigate the purification of flavonoids and alkaloids from the leaf of Nelumbo nucifera Gaertn by macroporous adsorption resin.Method Different types of macroporous adsorption resin were used for adsorpting.Spectrophotometry was used to measure the total flavonoids and alkaloids,and HPLC was used to determine the content of nuciferine.Result The D-101 macroporous adsorption resin was suitable for the purification of flavonoids and alkaloids from the leaf of Nelumbo nucifera Gaertn.Under the optimized conditions,the recovery rate of nuciferine was 57.2%.Conclusion This method was suitable for the purification of flavonoids and alkaloids from the leaf of Nelumbo nucifera Gaertn.

关 键 词：荷叶 黄酮 生物碱 大孔树脂 

分 类 号：R284[医药卫生—中药学]