心肌组织特异性微小RNA-1在ST段抬高型急性心肌梗死患者尿中含量的变化研究  

Urine cardiac specific microRNA-1 level in patients with ST segment elevation acute myocardial infarction

作　　者：段晓霞[1] 周贤[2] 王晓斌[1] 范忠才[1] 张春祥 陈庄[2] 

机构地区：[1]泸州医学院附属医院内科教研室,四川646000 [2]泸州医学院附属医院分子中心实验室,四川646000 [3]美国拉什大学药理系,芝加哥60612

出　　处：《中国危重病急救医学》2012年第12期709-712,共4页Chinese Critical Care Medicine

基　　金：四川省科技厅国际合作项目（2011HH0031）;美国NIH项目（HL080133.HL095707）

摘　　要：目的观察心肌组织特异性表达的微小RNA-1（microRNA-1，miR-1）在sT段抬高型急性心肌梗死（STEAMI）患者尿中的变化，探讨尿miR-1在STEAMI诊断中的潜在作用。方法20例STEAMI患者在发病12h内取尿，用实时荧光定量逆转录-聚合酶链反应（qRT-PCR）检测miR-l含量，同时检测血清心肌肌钙蛋白I（cTnI）和肌酸激酶同工酶（CK-MB）水平；以20例健康自愿者作为对照。结果STEAMI患者血cTnI（μg／L）、CK-MB（μg/L）和尿miR-1的含量[阈值循环（Ct）值，ct值越低，表示含量越高]均明显高于健康对照组（血cTnI：19．27±7．53比0．02±0．01，血CK-MB：93．82±12．30比0．86±0．63，尿miR-1：45．50±4．21比52．63±4．41，P〈0．05或P〈0．01）。当STEAMI患者血CK-MB〈300μg／L及cTnI〈50μg/L，尿miR-1含量与CK-MB、cTnI值呈直线相关（尿miR-1的ct值与血CK-MB：r=-0．81，P〈0．01；尿miR-1的ct值与血cTnI：r--0．63，P〈0．05）。结论尿miR-l可作为STEAMI早期诊断的新型敏感生物学标志物。Objective To observe the change of urine level of cardiac specific microRNA-1 （miR-1） in patients with ST segment elevation acute myocardial infarction （STEAMI） and investigate its potential applications. Methods Urine samples were collected from 20 STEAMI patients within 12 hours after STEAMI and from 20 healthy volunteers as control. Urine miR-1 concentrations were measured with real-time quantity reverse transcription-polymerase chain reaction （qRT-PCR）, at the same time serum cardiac troponin I （cTnI） and MB isoenzyme of creatine kinase （CK-MB） concentrations were measured. Results Serum level of cTnI （μg/L）, CK-MB （Ixg/L） and urine level of miR-1 in STEAMI patients were obviously higher than those in healthy control group [cTnI in blood： 19.27 ±7.53 vs. 0.02 ± 0.01, CK-MB in blood： 93.82 ± 12.30 vs. 0.86 ±0.63, miR-1 in urine （Ct value）： 45.50 ± 4.21 vs. 52.63 ± 4.41, P〈0.05 or P〈0.011. The urine miR-1 level in patients with STEAMI had a strong correlation with serum CK-MB or cTnI when CK-MB 〈 300 μg/L and cTnI 〈50 μg/L （Ct value of urine miR-1 with blood CK-MB： r=-0.81, P〈0.01 ; Ct value of urine miR-1 with blood cTnI： r=-0.63, P〈0.05）. Conclusion The results suggest that urine miR-1 could be a novel sensitive biomarker the early diagnosis of SETAMI.

关 键 词：ST段抬高型急性心肌梗死 微小RNA-1 心肌肌钙蛋白I 肌酸激酶同工酶 

分 类 号：R542.22[医药卫生—心血管疾病]