ST段抬高型急性心肌梗死患者外周血心肌特异性微小RNA-208a水平的变化  

Changes in cardiac specific microRNA-208a level in peripheral blood in ST segment elevation acute myocardial infarction patients

作　　者：姚怡[1] 周贤[2] 王晓斌[1] 段晓霞[1] 张春祥 史小玲[3] 陈庄[3] 范忠才[2] 

机构地区：[1]泸州医学院附属医院麻醉与危重病教研室,四川646000 [2]泸州医学院附属医院内科教研室,四川646000 [3]泸州医学院附属医院分子中心实验室,四川646000 [4]美国新泽西医学院麻醉与危重病教研室

出　　处：《中国危重病急救医学》2012年第12期717-720,共4页Chinese Critical Care Medicine

基　　金：四川省科技厅国际合作项目（2011HH0031）;美国NIH项目（HL080133,HL095707）

摘　　要：目的观察心肌特异性微小RNA-208a（microRNA-208a，miR-208a）在sT段抬高型急性心肌梗死（sTEAMI）患者血中的含量变化，探讨血miR-208a在STEAMI诊断中的作用。方法采集30例STEAMI患者发病12h内的外周血，用实时荧光定量逆转录-聚合酶链反应（qRT—PCR）检测血中miR-208a相对含量，同时用酶联免疫吸附试验（ELISA）检测血清心肌肌钙蛋白I（cTnI）、肌酸激酶同工酶（CK—MB）含量。以30例健康者血标本作为对照。结果STEAMI患者血cTnI（μg／L）、CK—MB（μg／L）含量明显高于健康对照者（cTnI：17．72±8．43比0．05±0．01，CK—MB：250．834-177．26比71．20±20．50，均P〈O．01）。在PCR60个循环内，健康对照者血均未能检出miR-208a，STEAMI患者血中可检出miR-208a（0-6h为44．95±4．77，6—12h为43．98±4．68）；以健康对照者血miR-208a阈值循环（ct）值60为基础进行统计，AMI患者血miR-208a相对含量至少高于健康对照者2-5倍以上，两组比较差异有统计学意义（P〈0．01）。结论血miR-208a可作为STEAMI患者早期诊断的新型敏感生物学标志。Objective To observe serum cardiac specific microRNA-208a （miR-208a） levels in ST segment elevation acute myocardial infarction （STEAMI） patients, and to explore the role of serum miR-208a levels in the diagnosis of STEAMI. Methods The serum miR-208a concentrations were assessed within 12 hours after STEAMI, while 30 healthy individuals as control. Serum miR-208a concentrations were measured with real-time quantity reverse transcription-polymerase chain reaction （qRT-PCR）, and serum cardiac troponin I （cTnI） or MB isoemzyme of creatine kinase （CK-MB） concentrations were measured with enzyme linked immunosorbent assay （ELISA）. Results The contents of serum cTnI （μg/L） or CK-MB （μg/L） in STEAMI patients were significantly higher than those in healthy individuals （cTnI： 17.72 ± 8.43 vs. 0.05 ± 0.01, CK-MB： 250.83 ± 177.26 vs. 71.20 ± 20.50, both P〈0.01 ）. Serum miR-208a concentrations were detected in all individuals with STE AMI within 60 PCR cycle （0-6 hours： 44.95 ± 4.77, 6-12 hours： 43.98 ± 4.68）, but were beyond detection for all individuals in the healthy control group. The serum miR-208a relative levels in STEAMI patients were at least more than 2is fold than that in healthy persons, compared with qRT-PCR （Ct =60） of miR-208a in healthy control persons （P 〈0.01 ）. Conclusion Serum miR-208a may be a new biomarker the early diagnosis of STEAMI patients.

关 键 词：ST段抬高型急性心肌梗死 微小RNA-208a 心肌肌钙蛋白I 肌酸激酶同工酶 

分 类 号：R542.22[医药卫生—心血管疾病]