丙泊酚预处理对谷氨酸损伤大鼠脑组织的保护作用研究  

The protective effect of propofol pretreatment on glutamate injury of neonatal rat brain slices

作　　者：周晓峰[1] 黄丁丁[2] 王迪芬[1] 付江泉[1] 

机构地区：[1]贵阳医学院附属医院重症医学科,贵州550004 [2]上海市第六人民医院麻醉科

出　　处：《中国危重病急救医学》2012年第12期750-753,共4页Chinese Critical Care Medicine

基　　金：国家临床重点专科建设项目（2010SY3079）;贵州省科技攻关计划项目（SY20103079）;贵州省贵阳市科技攻关项目（[2010]筑科农合同字第1-社-16号）

摘　　要：目的探讨丙泊酚预处理对谷氨酸（Glu）损伤大鼠脑组织的保护作用。方法取出生10～15dsD大鼠脑皮质切片进行培养，观察脑片形态学变化。将脑皮质切片分为空白对照组、Glu损伤组（1mmol／LGlu作用0．5h）及丙泊酚预处理组（损伤前给予20mg／L丙泊酚作用24h），每组12个样本。镜下观察各组脑皮质切片的细胞病理改变及超微结构变化，计算乳酸脱氢酶（LDH）漏出率，免疫组化法检测胶质纤维酸性蛋白（GFAP）阳性表达并计数。结果培养的脑皮质切片细胞形态完整、存活良好。苏木素一伊红（HE）染色、电镜及LDH检测结果显示：Glu损伤组脑皮质切片中神经元细胞损伤严重，形态不规则，胶质细胞增生、水肿，LDH漏出率较空白对照组明显增高（（68．5±2．0）％比（16．0±2．5）％，P〈0．01]；丙泊酚预处理组脑皮质切片神经元细胞损伤减轻，细胞形态恢复，LDH漏出率较Glu损伤组明显减少[（38．5±2．4）％比（68．5±2．0）％，P〈0．05]。免疫组化检测结果显示：Glu损伤组胶质细胞胞体肿胀，突起数量增多，GFAP阳性反应强，阳性细胞数量（个，HP）较空白对照组显著增多（50±5比10±3，P〈0．01）；丙泊酚预处理组胶质细胞形态有所恢复，细胞突起细长，GFAP阳性反应减弱，阳性细胞数量较Glu损伤组明显减少（30±4比50±5，P〈0．05）。结论丙泊酚预处理对Glu损伤的SD大鼠脑皮质神经元具有保护作用。0bjective To study the protective effect of propofol precondition against glutamate （Glu） neurotoxicity to neonatal rat cerebrocortical slices. Methods Brain slices of Sprague-Dawley （SD） rats were cultured in vitro and observed the morpholgic changes. Brain slices were randomly divided into three groups： blank control group, Glu injury group （ 1 mmol/L Glu for 0.5 hour）, propofol precondition group （20 mg/L propoful for 24 hours）, each n = 12. Changes in pathological and ultra-structure of cells were observed using microscope. Lactate dehydrogenase （LDH） leakage rate was measured. Meanwhile, the expression of glial fibrillary acidic protein （GFAP） was detected by immunohistochemical technology, then the positive cell were counted. Results Cultured brain slices of cell were intact and survived well. Hematoxylin-eosin （HE） staining, electron microscopy and LDH test results showed that cerebral film neuron severely damage, gliosis, edema, LDH leakage rate in Glu injury group were significantly more severe compared with blank control group （68.5 ± 2.0）% vs. （ 16.0 ± 2.5）%, P〈0.01 ]. Reduce the brain slice of the propofol pretreatment group of neuronal cell jury, cell shape recovery significantly reduced LDH leakage rate compared with the Glu injury group （38.5 ± 2.4）% vs. （68.5 ± 2.0）%, P〈0.051. Immunohistochemical detection of GFAP expression of Glu injury group glial cell body swelling, producing increase in the number of GFAP positive reaction strong, the number of positive ceils （cells/HP） compared with blank control group was significantly increased （50 ± 5 vs. 10 ± 3, P〈0.01 ）. The recovery of propofol pretreatment group glial cell morphology, cell processes slender GFAP positive reaction decreased the number of positive cells compared with the Glu injury group was significantly decreased （30 ± 4 vs. 50 ± 5, P〈0.05 ）. Conclusion Propofol pretreatment haprote ctive effect against Glu injuryed rat cerebrocortical slices.

关 键 词：丙泊酚 预处理 谷氨酸 脑损伤 胶质纤维酸性蛋白 

分 类 号：R965[医药卫生—药理学]