Analysis and Evaluation Indicator Selection of Chilling Tolerance of Different Cotton Genotypes  

不同棉花基因型幼苗耐寒性分析及其鉴定指标筛选(英文)

作　　者：武辉[1] 侯丽丽[1] 周艳飞[1] 范志超[1] 石俊毅[1] 阿丽艳.肉孜 张巨松[1] 

机构地区：[1]新疆农业大学农学院/教育部棉花工程研究中心,新疆乌鲁木齐830052

出　　处：《Agricultural Science & Technology》2012年第11期2338-2346,共9页农业科学与技术（英文版）

基　　金：Supported by"11thFive-Year Plan"National Science and Technology Support Program(2009BADA4B01-3)~~

摘　　要：[Objectivc] This study aimed to investigate the chilling tolerance of seedlings of different cotton genotypes and screen appropriate indicators for assess- ing chilling tolerance, to establish reliable mathematical evaluation model for chilling tolerance of cotton, thus providing theoretical basis for breeding and promoting new chilling-tolerant cotton germplasms and large-scale evaluation of chilling tolerance of cotton varieties. [Method] Fifteen cotton varieties （lines） were used as experimental materials. The photosynthetic gas exchange parameters, chlorophyll fluorescence ki- netic parameters, chlorophyll content, relative soluble sugar content, malonaldehyde content, relative proiine content, relative conductivity and other 12 physiological indi- cators of seedling leaves under low temperature treatment （5 ℃, 12 h） and recovery treatment （25 ℃. 24 h） were determined; based on the chilling tolerance coefficient （CTC） of various individual indicators, the comprehensive evaluation of chilling toler- ance was conducled by using principal component analysis, hierarchical cluster anal- ysis and stepwise regression analysis. [Result] The results showed that the 12 indi- vidual physiological indicators could be classified into 7 independent comprehensive components by principal component analysis; 15 cotton varieties （lines） were clus- tered into three categories by using membership function method and hierarchical cluster analysis; the mathematical model for evaluating chilling tolerance of cotton seedlings was established： D =0.275 -0.244Fo1 ＋0.206Fv/Fm1＋0.326g,%-0.056SS ＋ 0.225MDA＋O.O38REC （FF=0.995）, and the evaluation accuracy of the equation was higher than 94.25%,0. Six identification indicators closely related to chilling tolerance were screened, including Fo,, Fv/Fm1, Seedling leaves of cotton varieties （lines） gs2, SS, MDA, and REC. [Conclusion] with high chilling tolerance are less dam- aged under low temperature stress, and are able to maintain relatively high p[目的]研究不同基因型棉花幼苗耐寒特性,筛选耐低温鉴定指标,建立可靠的棉花耐寒性数学评价模型,为棉花耐寒新品种选育、推广及大规模品种耐寒性评价奠定基础。[方法]以15个棉花品种(系)为试验材料,对其在低温胁迫(5℃、12h)及恢复处理(25℃、24h)下幼苗叶片的光合气体交换参数、叶绿素荧光动力学参数、叶绿素含量、可溶性糖含量、丙二醛含量、游离脯氨酸含量和相对电导率等12个生理指标进行测定,以各单项指标的耐寒系数作为衡量耐寒性的依据,运用主成分分析、聚类分析和逐步回归等方法对其耐寒性进行综合评价。[结果]通过主成分分析将12个单项生理指标转换为7个相互独立的综合指标;通过隶属函数法和聚类分析将15个棉花品种(系)按耐寒性强弱划分为3类;通过逐步回归建立棉花幼苗耐寒性评价数学模型,D=0.275-0.244Fo1+0.206Fv/Fm1+0.326gs2-0.056SS+0.225MDA+0.038REC(R2=0.995),估计精度大于94.25%,并筛选出6个耐寒性鉴定指标,分别是Fo1、Fv/Fm1、gs2、SS、MDA和REC。[结论]耐寒性强的棉花品种(系)幼苗叶片在低温胁迫下受到伤害较轻,能保持较高的光合电子传递能力,经常温恢复后叶片气孔导度较高,便于光合气体交换,有助于光合能力的恢复;在相同逆境下,通过测定Fo1、Fv/Fm1、gs2、SS、MDA和REC等6个鉴定指标,可进行棉花品种耐寒性强弱的快速鉴定和预测。

关 键 词：COTTON Chilling tolerance Principal components analysis Comprehensiveevaluation Stepwise regression analysis 

分 类 号：S562[农业科学—作物学] S511