山莨菪中乙酰胆碱酯酶抑制剂的分离  

Isolation of Acetylcholinesterase Inhibitors from Anisodus Tangutcus

作　　者：杨中铎[1] 薛鹏辉[1] 

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《中医药学报》2012年第5期19-21,共3页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金资助项目(20802031)

摘　　要：目的:从山莨菪根部的总生物碱提取物中分离乙酰胆碱酯酶抑制剂。方法:山莨菪根经95%乙醇提取、4mol/L HCl和4mol/L NaOH酸碱处理,氯仿萃取,得到总生物碱。采用薄层色谱生物自显影法跟踪分离目标组分,再用改良的Ellman法进一步测定抑制率。利用多种柱色谱分离得到3个化合物(1-3),通过1H-NMR、13C-NMR、ESI-MS波谱方法并与文献比对鉴定化合物的结构。结果:从山莨菪根部的总生物碱提取物中分离得到3个已知化合物,它们分别被鉴定为东莨菪碱,樟柳碱,阿托品,其乙酰胆碱酯酶抑制率分别为45.28%,38.08%和25.10%。结论:莨菪碱衍生物具有较弱的乙酰胆碱酯酶抑制活性。Objective ： To isolate acetylcholinesterase inhibitor from total alkaloidal extracted of the roots of Anisodus tan- guteus. Method ： The root of Anisodus tangutcus were extracted by 95 % ethanol and treated with 4 mol · L^-1HCl and 4 mol · L^-1 NaOH , and then extracted by chloroform to obtain total alkaloidal extracts. The bioassay -guided isolation of the AChE inhibitor was executed by TLC bioautographic method , and modified Ellman＇ s colorimetric method in 96 - well microplates. Three compounds （ 1 - 3 ） were obtainded by various column chromatographic methods and their struc- tures were elucidated based on1HNMR, 13 C - NMR , ESI - MS analysis and compared with literature reports. Result ： Three known compounds were isolated and identified as scopolamine, anisodine, atropine, respectively. Their inhibitory rates of 1 -3 against AchE were 45.28%, 38.08% and 25.10%, respectively. Conclusion： Derivatives of hyoseyamine have a weak inhibitory activity to AchE.

关 键 词：山莨菪 乙酰胆碱酯酶抑制剂 总生物碱 

分 类 号：R284.2[医药卫生—中药学]