促孕合剂的质量标准研究  

Study on the Quality Standard for Cuyun Mixture

作　　者：邓少荣[1] 曾建武[1] 李靖[2] 吴云霞[1] 

机构地区：[1]华中科技大学同济医学院药学院,武汉430030 [2]武警湖北总队医院,武汉430060

出　　处：《中国实验方剂学杂志》2012年第23期64-66,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30801538)

摘　　要：目的:建立促孕合剂的质量标准。方法:采用薄层色谱法(TLC)对处方中黄芪,黄芩,续断进行定性鉴别;用高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定黄芪甲苷的含量,采用Kromasil C18柱,流动相乙腈-水(35∶65),流速0.8 mL·min-1。结果:薄层色谱专属性强,黄芪甲苷的线性范围1.02～10.2μg(r=0.999 7),平均回收率为99.96%,RSD1.01%,(n=6)。结论:该方法简便、准确、灵敏度高,可用于促孕合剂的质量控制。Objective： To establish the quality standard for Cuyun mixture. Method： Radix Astragali Mongolici, Radix Scutellariae Baicalensis and Radix Dipsaci in this prescription were identified by TLC; and the content of astragaloside IV in Cuyun mixture was determined by HPLC. The column of Kr0masil Cls （4.6 mm × 250 mm, 5 μm） was used; the mobile phase consisted of acetonitrile-water （35：65） ; the flow rate was 0. 8 mL· min^-1. Result： TLC spots were clear with strong specificity and easy identification. While the linear range of astragaloside IV was 1.02-10. 2 μg （r = 0. 999 7）, the average recovery rate was 99.96% , RSD 1.01% （n = 6）. Conclusion： The method is easy, accurate and reproducible. It can be used effectively for the quality control of this preparation.

关 键 词：促孕合剂 黄芪甲苷 高效液相色谱法 质量标准 

分 类 号：R284.1[医药卫生—中药学]