HPLC测定梓木草饮片中尿囊素的含量  

Determination of Allantoin in Lithospermun Zollingeri by HPLC

作　　者：徐志红[1,3] 贾晓斌[1,2] 陈磊垚[3] 

机构地区：[1]南京中医药大学,南京210046 [2]江苏省中医药研究院中药新型给药系统重点实验室,国家中医药管理局中药释药系统重点研究室,南京210028 [3]南京市中西医结合医院,南京210014

出　　处：《中国实验方剂学杂志》2012年第23期112-114,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立梓木草饮片中尿囊素含量测定的方法。方法:采用HPLC测定。色谱柱Hypersil ODS-C18柱(4.6mm×250 mm,5μm),流动相甲醇-水(5∶95),流速0.2 mL·min-1,检测波长210 nm,柱温30℃,进样量20μL。结果:尿囊素在12.32～61.60μg呈良好的线性关系(r=0.999 9);尿囊素平均加样回收率为98.1%,RSD 1.55%(n=6)。结论:该方法可以作为梓木草饮片的质量控制,不同产地梓木草饮片中尿囊素含量差异较大。Objective ： To establish a method for the determination of allantoin in Lithospermun zollingeri. Method： Separation and determination of allantoin were accessed by using an ODS-C18 column and a mobile phase of methanol-water at a ratio of 5 ： 95. The flow rate was 0.2 mL · min^-1, temperature at 30 ℃ and detection wavelength at 210 nm. Result： A good linearity of allantoin was achieved in the range of 12.32-61.60 μg （r = 0. 999 9） . The mean value of recovery was 98.1% , RSD was 1.55%. Conclusion： The method in this paper could provide an evidence for quality control of L. zoUingeri and there is comparatively large difference of content of allantoin in L. zoUingeri from different producing areas.

关 键 词：梓木草 HPLC 尿囊素 含量测定 

分 类 号：R284.1[医药卫生—中药学]