通心络对异丙肾上腺素诱导的H9c2心肌细胞肥大及Cx43mRNA表达的影响  

Effect of Tongxinluo on H9c2 Cardiomyocytes Hypertrophy and Expression of Connexin43 mRNA Induced by Isoproterenol

作　　者：杨军[1] 丁赛良[1] 邓彪[1] 王光辉[1] 张勇[1] 王苏燕[1] 邝孛[1] 

机构地区：[1]南华大学附属第一医院心内科,湖南衡阳421001

出　　处：《中国实验方剂学杂志》2012年第23期191-194,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖南省科技厅重点课题项目(2009SK2010);湖南省自然科学基金重点项目(11JJ2046)

摘　　要：目的:观察通心络(Tongxinluo,TXL)超细干粉水提物对异丙肾上腺素(isoproterenol,Iso)诱导的H9c2心肌细胞肥大的作用,并探讨其对缝隙连接蛋白43(connexin 43,Cx43)mRNA表达的影响。方法:将培养的H9c2心肌细胞随机分为对照组、TXL组、Iso组和Iso+TXL组,通心络以通心络超细干粉水提物的形式加入,使用异丙肾上腺素诱导72 h后,采用相差显微镜测量细胞大小,BCA法测定蛋白浓度,运用逆转录聚合酶链式反应(RT-PCR)测定Cx43mRNA的表达。结果:Iso刺激H9c2心肌细胞72 h后,Iso组细胞直径增大,高于对照组(P<0.05);通心络对正常H9c2心肌细胞直径无明显影响,但能抑制Iso诱导的细胞直径增大(P<0.05)。Iso组蛋白浓度明显升高,高于对照组(P<0.05);通心络对正常H9c2心肌细胞蛋白浓度无明显影响,但能抑制Iso诱导的蛋白浓度的升高(P<0.05)。Iso组Cx43mRNA表达明显减少,低于对照组(P<0.05);通心络对正常H9c2心肌细胞Cx43mRNA表达无影响,但能够抑制Iso诱导的Cx43 mRNA表达的下调(P<0.05)。结论:通心络可以抑制Iso诱导的H9c2心肌细胞肥大及Cx43mRNA表达的下调。Objective： To investigate the effects of Tongxinluo （TXL） on hypertrophy of H9c2 cardiomyocytes and connexin 43 mRNA expression induced by isoproterenol （Iso）. Method： Cultured H9c2 cardiomyocytes were randomly divided into four groups： control group, TXL group, Iso group, and Iso ＋ TXL group. After 72 hours of culture, cell size was determined by phase contrast microscope, the protein concentrations were measured by BCA method and the expression of Cx43 mRNA were measured by reverse transcription polymerase chain reaction （RT-PCR）. Result： Cell size of H9c2 cardiomyocytes of the Iso group （P 〈0.05） was increased more prominently than the control group. Compared with Iso group, Tongxinluo attenuated the increase of cardiomyocytes size （P 〈 0.05）. H9c2 cardiomyocytes protein concentrations in the Iso group （P 〈 0.05） was increased more significantly than the control group. Although Tongxinluo didn＇t affect the normal H9c2 cardiomyocytes protein concentration, it inhibited the increase of protein concentrations induced by Iso （P 〈 0.05）. Iso decreased Cx43 mRNA expression of H9c2 cardiomyocytes more evidently than that of the control group （P 〈 0.05）, which was inhibited by Tongxinluo （P 〈 0.05）. Conclusion： Tongxinluo can prevent the hypertrophy of H9c2 cardiomyocytes and down-regulate of Cx43 mRNA expression induced by Iso.

关 键 词：通心络 心肌肥大 H9C2心肌细胞 连接蛋白43 异丙肾上腺素 

分 类 号：R285.5[医药卫生—中药学]