辛开苦降法颗粒剂对KKay2型糖尿病小鼠胰岛素敏感性的影响  

Effect of Xinkai kujiang Formula Granules on Insulin Sensitivity in KKay Type 2 Diabetic Mice

作　　者：柳红芳[1] 胡照娟[1] 张艳红[1] 郭会霞[1] 

机构地区：[1]北京中医药大学东直门医院内分泌科,北京100700

出　　处：《中国实验方剂学杂志》2012年第23期233-237,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072735);北京中医药大学自主创新项目(2009JYB22-JS077);首都特色基金项目(Z111107058811058)

摘　　要：目的:运用转基因Kkay2型糖尿病小鼠为模型,观察用辛开苦降法传统煎剂(辛开苦降第1组)、辛开苦降法颗粒剂(辛开苦降第2组)干预对Kkay 2型糖尿病小鼠胰岛素敏感性及血脂的疗效。方法:10周龄雄性初发2型糖尿病KKay小鼠36只按血糖值随机分为4组:模型组、罗格列酮组(0.67 g·kg-1·d-1)、辛开苦降煎剂组(8.7 g·kg-1·d-1)、辛开苦降颗粒剂(8.7 g·kg-1·d-1)。每天灌胃给药1次,4周后测定血糖、空腹血糖、胰岛素、血脂,计算胰岛素敏感指数。结果:给药4周后,与正常组相比,模型组胰岛素敏感指数明显降低(P<0.01),空腹血糖、甘油三酯、总胆固醇等明显升高。辛开苦降煎剂、辛开苦降颗粒剂与模型组相比,胰岛素敏感指数高于模型组(P<0.01),空腹血糖值低于模型组(P<0.01);甘油三酯、总胆固醇均低于模型组,辛开苦降传统煎剂和辛开苦降法颗粒剂之间并无差异。结论:辛开苦降法可改善初发2型糖尿病KKay小鼠的胰岛素抵抗,增加胰岛素的敏感性从而改善血糖水平,并能降低血脂水平,且煎剂和颗粒剂疗效相当。Objective： Using genetically modified Kkay type 2 diabetic mice as the model, to observe the effect of Xinkaikujiang traditional decoction （XKKJ-group 1 ） and Xinkaikujiang Formula granules （XKKJ-group 2） for insuhn sensitivity and blood lipids in KKay type 2 diabetic mice. Method： According to the blood sugar, thirty- six male KKay mice （10 weeks old ） were randomly divided into 4 groups, including the model group, the rosiglitazone group （0.67 g · kg^-1 · d^-1 ）, the XKKJ-group 1 and the XKKJ-group 2 （8.7 g · kg^-1 · d^-1 ）. The control group was consisted of 9 C57BL/6J male mice （10 weeks old）. The treatment groups were administered orally once a day＇ and both the control group and model group were given 0. 5% sodium carboxymethylcellulose （CMC） solution. After 4-week treatment, the random blood sugar, fasting blood sugar （FBG） , serum insulin and blood lipids were determined, and the insulin sensitivity index was calculated. Result： After 4-week treatment, compared with the control group, the model group＇s ISI was significantly lowered （ P 〈 0.01 ）, FBG, triglyceride （TG） and total cholesterol （TC） were increased significantly （P 〈 0. 01 ）. Compared with the model group, ISI in XKKJ-group 1 and XKKJ-group 2 was significantly improved （P 〈0.01）, FBG significantly lowered （P 〈0. 01 ） TG and TC lowed. There were no difference between XKKJ-group 1 and XKKJ-group 2. Conclusion： The Chinese medicine of XKKJ could improve the insulin resistance in KKay type 2 diabetes mice, increase insulin sensitivity and rnduce the blood sugar level, lipid levels. The efficacy is not related to the dosage form.

关 键 词：辛开苦降法 KKay2型糖尿病小鼠 胰岛素敏感性 血糖 血脂 

分 类 号：R285.5[医药卫生—中药学]