剪应力联合参莲提取物对血管内皮细胞炎症蛋白表达的影响  

Shear Stress Combined with Shenlian Extracts Regulates E-selectin Expression via NF-κB Pathway in Endothelial Cells

作　　者：游云[1] 龚曼[1] 李玉洁[1] 郭宇飞[1] 杨洪军[1] 廖福龙[1] 张根明[2] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]北京中医药大学东直门医院,北京100700

出　　处：《中国实验方剂学杂志》2012年第23期261-265,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30801530);中国中医科学院自主课题(ZZ20090109)

摘　　要：目的:观察流动剪应力联合参莲提取物对血管内皮细胞炎症通路E选择素(E-selectin)及核因子NF-κB的影响。方法:本研究采用生物力药理学的研究方法,2×2析因设计分组,利用BioFlux 1000控剪应力微流培养系统,以2水平剪应力(1,10 dyn·cm-2)联合2剂量参莲提取物对人脐静脉血管内皮细胞(HUVECs)进行预处理10 h,以免疫荧光法分析流动剪应力与药物对肿瘤坏死因子-α(TNF-α)引起的内皮炎症蛋白表达的影响。结果:剪应力与药物在抑制内皮细胞膜表达E-selectin方面,具有交互作用,10 dyn·cm-2剪应力可以显著提高药效。作为单因素,流动剪应力及参莲提取物均可抑制TNF-α诱导的血管内皮细胞NF-κB表达,二者未见交互作用。结论:血流剪应力可影响药效,参莲提取物与正常血流剪应力联合作用可通过NF-κB路径抑制E-selectin表达。Objective： To observe the joint effect of shear stress and Shenlian （SL） extracts on E- selectin and neuclear factor KB （NF-KB） expression in endothelial cells. Method： 2 × 2 factorial design was employed to analyze the interactions between shear stress and SL extracts. Biofluxl000 microfluidic device was used to control two levels of shear stress. E-selectin and NF-KB expression stimulated by tumor neerosis factor -α （TNF- α） in human umbilical vein endothelial cells （HUVECs） were detected by using immunofluorescence method after pretreatment 10 hours with different level, of shear stress and SL extract. Result： There was an interaction associated with each factor in regulating E-selectin expression. Combined treatment of SL extract with 10 dyn ·cm^-2 shear stress led to a synergistic decrease in E-selectin expression in endothelial cells. As a single factor, SL extract or shear stress alone could decrease NF-KB expression significantly. Conclusion： Shear stress may influence the SL extract＇s atheroprotective effect through downregulation of E-selectin via NF-KB in endothelial cells.

关 键 词：剪应力 参莲提取物 人脐静脉血管内皮细胞 血管内皮细胞 生物力药理学 

分 类 号：R285.5[医药卫生—中药学]