柴胡-白芍药对抗抑郁作用的实验研究  

Study on the Antidepressant-like Effect of Bupleuri Radix and Paeoniae Radix Alba in Different Doses

作　　者：于春泉[1] 李苒[1] 张敏[1] 高杉[1] 王怡[1] 

机构地区：[1]天津中医药大学,天津300193

出　　处：《中国实验方剂学杂志》2012年第23期286-289,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究不同剂量柴胡-白芍药对及单用柴胡、白芍的抗抑郁作用。方法:雄性ICR小鼠按体重随机分为空白对照组、阳性药对照组(盐酸氟西汀0.015 g·kg-1)及柴胡-白芍(1∶1)药对高、中、低剂量组(32,16,8 g·kg-1),柴胡高、中、低剂量组(32,16,8 g·kg-1),白芍高、中、低剂量组(32,16,8 g·kg-1)共计11组。各组小鼠灌胃给药7 d后采用小鼠开场实验(OFT)排除假阳性结果,并通过小鼠悬尾实验(TST)、小鼠强迫游泳实验(FST)研究各药的抗抑郁作用。结果:在OFT中,各组小鼠实验结果无显著差异。在TST和FST中,各给药组小鼠的不动时间均显著减少,其中以柴胡-白芍药对高剂量组对小鼠不动时间的影响较为明显,在TST中不动时间为(51.3±31.7)s,在FST中不动时间为(86.3±25.9)s,分别与空白组比较均有显著性差异(P<0.01),并且TST和FST的结果具有一致性。结论:不同剂量的柴胡-白芍药对及单用柴胡、白芍均具有明显的抗抑郁作用,但它们抗抑郁作用强度存在差异。Objective： To study the antidepressant-like effect of the coupled drug dispensing of Bupleuri Radix and Paeoniae Radix Alba, single use of Bupleuri Radix and single use of Paeoniae Radix Alba in different doses. Method： Male ICR mice were divided into 11 groups： control group, fluoxetine （0. 015 g.·kg^-1） group, the coupled drug dispensing of Bupleuri Radix and Paeoniae Radix Alba （ 1 ： 1 ） in high, middle and low doses （32, 16, 8 g ·kg^-1） groups, single use of Bupleuri Radix in high, middle and low doses （32, 16, 8 g·kg^-1） , single use of Paeoniae Radix Alba in high, middle and low doses （32, 16, 8 g ·kg^-1） groups. The open field test （OFT） was used to exclude false-positive results at 7 d after the administration （ig）. And the tail suspension test （TST） and the forced swimming test （FST） were used to study the antidepressant-like effect of different groups. Result： There was no significant difference in different groups in the experiment of OFT. Different groups reduced the immobility time both in the TST and FST. Most obviously in the coupled drug dispensing of Bupleuri Radix and Paeoniae Radix Alba （1： 1） in high doses （32 g·kg^-1） , in the TST, the immobility time was （51.3 ± 31.7） s, and in the FST, the immobility time was （86.3 ± 25.9） s. Compared with the control group, the coupled drug dispensing of Bupleuri Radix and Paeoniae Radix Alba （1：1 ） in high＇doses （32 g·kg^-1） had significant difference in TST and FST test （P 〈 0.01 ）. In addition, the results of the TST were in agreement with those in the FST. Conclusion： The results suggested that the coupled drug dispensing of Bupleuri Radix and Paeoniae Radix Alba, single use of Bupleuri Radix and single use of Paeoniae Radix Alba in different doses performed antidepressant-like effect, while there was difference in the antidepressant-like effect.

关 键 词：柴胡 白芍 抗抑郁 小鼠悬尾实验 小鼠强迫游泳实验 

分 类 号：R285.5[医药卫生—中药学]