W16转济麦19小麦株系抽穗期抗旱性研究  被引量:4

Drought resistance of transgenic wheat with W16 gene during heading stage

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作  者:王刚[1] 刘曙东[1] 闽东红[1] 王竹林[1] 杨兴圣[1] 李华[1] 徐兆师[2] 陈明[2] 李连城[2] 马有志[2] 

机构地区:[1]西北农林科技大学农学院,陕西杨凌712100 [2]中国农业科学院作物科学研究所/国家农作物基因资源和基因改良重大科学工程/农业部作物遗传改良与育种重点开放实验室,北京100081

出  处:《干旱地区农业研究》2012年第6期1-6,共6页Agricultural Research in the Arid Areas

基  金:转基因生物新品种培育科技重大专项(2009ZX08002-008B)

摘  要:以两个转W16基因小麦株系及相应的受体对照品种为材料,在大田控水的条件下研究了水分胁迫对转基因小麦抽穗期相关生理特性的影响,利用方差分析方法对转基因株系的抗旱性进行了综合评价。结果表明:转基因株系和对照及受体有着较大的差异,随着水分胁迫的加剧,参试品种的相对含水量、气孔导度、光合速率、叶绿素含量呈下降趋势,转基因小麦的下降幅度较小;丙二醛含量、胞间CO2浓度、质膜相对透性呈上升趋势。受体对照品种的上升幅度大于转基因小麦;转基因小麦的脯氨酸含量显著高于对照品种。方差分析表明,转基因小麦株系G19-X51、G19-X61具有较好的抗旱性。Using two transgenic wheat strains with W16 gene and corresponding recurrent parent as materials. Field experiments were conducted under different water stress to compare drought-resistance of different varieties by studying the effect of physiological and biochemical indexes on drought resistance of transgenic wheat during heading stage, and com- bined with the method of analysis of variance of genetically modified strain drought-resistance of the comprehensive evalu- ation. The results showed that: genetically modified strain and control and receptor has obvious difference, with the increase of water stress, the relative water content, stomatal conductance, light and rate, chlorophyll content showed down- trend, and the transgenic wheat declined more slowly; MDA content, the intercellular CO2, plasma membrane permeability showed uptrend, but the corresponding contrast rose more steeply. The praline content of transgenic wheat was significantly higher than contrast varieties. The analysis of variance showed that transgenic whea strains G19 - XS1, G19 - X61 had strong drought resistance.

关 键 词:小麦 W16基因 水分胁迫 抗旱性 

分 类 号:S512.1[农业科学—作物学] S332.4

 

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