双歧醋对高脂饮食大鼠血脂影响的机制探讨  

The mechanism of bifidobacterium vinegar affecting the plasma lipid of rats fed with high-fat diet

作　　者：李金玲[1,2] 张德纯[1,2] 刘永云 陈翠竹 王春耀 

机构地区：[1]重庆医科大学病原生物学教研室 [2]分子医学与肿瘤研究中心,重庆400016

出　　处：《中国微生态学杂志》2012年第11期987-991,共5页Chinese Journal of Microecology

摘　　要：目的探讨双歧醋对高脂饮食大鼠血脂的影响机制。方法 48只SD大鼠被随机分成双歧醋低剂量组[1.8 mL/(kg.BW)]、中剂量组[3.4 mL/(kg.BW)]、高剂量组[6.8 mL/(kg.BW)]和市售醋组[3.4 mL/(kg.BW)]、高脂模型组以及基础对照组,观察双歧醋对高脂饮食大鼠血脂的影响作用机制,测定指标包括大鼠肝脏脂质、大鼠LDLR的免疫组化分析,HMGCoA还原酶和LDLR的RT-PCR分析。结果双歧醋各组对于HMGCoA还原酶表达有抑制作用,但可以上调LDLR的基因和蛋白质表达。结论双歧醋可以通过调节HMGCoA还原酶和LDLR的表达预防高脂饮食大鼠高血脂的发生。Objective To discuss the prophylactic antiobesity effect of bifidobacterium vinegar and its possible mechanism. Methods 48 SD rats were randomly divided into 6 groups ： bifidobaeterium vinegar groups of three different doses, ordinary vineger group, high-fat model group and basic group. The possible mechanism of bifidobacterium vinegar affecting the plasma lipid of rats fed with high-fat diet was observed. The lipidoses in liver were measured; mRNA expression of LDLR and HMGCoA reductase in liver tissue were detected by immunohistochemistry and RT-PCR. Results The levels of HMGCoA reduetase of bifidobacterium vinegar groups were depressed obviously compared with that of high-fat model group. Conclusion Bifidobacterium vinegar can prevent the oecurance of high-fat in rats through adjusting the LDLR and HMGCoA reductase in liver to some degree.

关 键 词：双歧醋 大鼠 高脂血症 机制 

分 类 号：R378.992[医药卫生—病原生物学]