一株低温产甲烷菌的筛选分离与系统发育分析研究  

Isolation and Phylogenetic Analysis of a Psychrophilic Methanogen

作　　者：张硕[1] 叶尔买克[2] 鲁建江[1] 

机构地区：[1]石河子大学化学化工学院/新疆兵团化工绿色过程重点实验室,石河子832003 [2]阿拉山口出入境检验检疫局技术中心,阿拉山口833418

出　　处：《石河子大学学报（自然科学版）》2012年第4期513-517,共5页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(21067011);新疆兵团重点科技攻关计划(2010GG21)

摘　　要：采用厌氧分离技术从新疆越冬沼气池分离出一株产甲烷菌株SZ-3,该菌株5～40℃范围内均能生长,其生长pH范围4～8,符合低温兼性嗜冷产甲烷菌筛选要求;该菌株最适生长温度20℃,生长最适pH值为7,最适NaCl浓度为0～0.4mol/L;该菌株革兰氏染色阳性,菌落呈黄色,显微镜观察为不规则球状,菌体聚集且不规则裂殖。菌株只能利用甲醇、H2CO2(80∶20)混合气、甲胺、二甲胺,三甲胺,不能利用甲酸、乙酸等生长;16SrDNA序列分析,SZ-3菌株和产甲烷八叠球菌属同源性最高为99%,系统发育分析和生理生化特征研究表明此菌株为甲烷八叠球菌属湖沉积产甲烷八叠球菌种(Methanosarcina lacustris)。A methanogen SZ-3 with growth temperature of 5~40℃,pH 4~8 and optimal growth conditions： 20℃,pH 7.0,0~0.4 mol/L NaCl,was isolated from the winter biogas digester of Xinjiang,by anaerobic screening technique,consistent with low temperature and the amphipsychrophilic methanogens screening requirements.The strain was gram-positive,irregular spherical,cell aggregation,and irregular fission.This strain could use hydrogen and carbon dioxide（80∶20）,or methylamine,dimethylamine,trimethylamine,methanol as substrates for its growth and methanogenesis,but it could not use formate acid and acetic acid.Sequence analysis of the 16S rRNA genes of strain SZ-3 and Methanosarcina revealed that the similarity reaches to 99%.Phylogenetic analysis and physicobiochemical characteristics show that strain SZ-3 belongs to Methanosarcina lacustris.

关 键 词：产甲烷菌 兼性嗜冷 富集培养 16SRDNA序列 

分 类 号：S216.4[农业科学—农业机械化工程]