心肌肥厚心力衰竭兔室性心律失常与氧化应激  

Oxidative stress and ventricular arrhythmias in rabbits with myocardial hypertrophy or heart failure

作　　者：贺莉[1] 肖建民 付晖[3] 杜广胜[4] 肖幸[4] 张存泰[4] 顾晔[1] 马业新[4] 

机构地区：[1]湖北省武汉市普爱医院心内科,湖北武汉430033 [2]广东省东莞太平医院心内科 [3]广东医学院药理系 [4]华中科技大学同济医学院附属同济医院心内科

出　　处：《中国心脏起搏与心电生理杂志》2012年第5期427-430,共4页Chinese Journal of Cardiac Pacing and Electrophysiology

基　　金：国家自然科学基金资助项目(项目编号:30971180);湖北省自然科学基金项目(项目编号:2009CDB127)

摘　　要：目的研究心肌肥厚和心力衰竭兔氧化应激的变化,并探讨室性心律失常与氧化应激的关系。方法 40只日本长耳白兔随机分为假手术(Sham 1、Sham 2)组、心肌肥厚(LVH)组、心力衰竭(HF)组,每组10只。LVH组和HF组通过缩窄腹主动脉制备LVH与HF模型,Sham 1组和Sham 2组仅游离腹主动脉不进行缩窄术。Sham 1组和LVH组喂养8周;Sham 2和HF组喂养20周。超声心动图观察各组心脏结构变化,检测兔血清中丙二醛(MDA)、超氧化物岐化酶(SOD)和NT-脑钠肽(NT-proBNP)水平,并制备兔左室楔形心肌块的灌注模型,观察在异丙肾上腺素(2μmol/L)灌注下,快频率程序刺激各组触发活动和室性心动过速(简称室速)或心室颤动(简称室颤)的发生率。结果与Sham 1组比较,LVH组室间隔厚度(IVST)明显增厚(P<0.05),血清SOD活性降低、MDA浓度增加,触发活动和室速或室颤发生率明显升高(P<0.05);与Sham 2组比较,HF组左室舒张末期(LVEDd)增大、IVST增厚、射血分数(LVEF)降低(P<0.05),血清SOD活性降低、NT-proBNP和MDA浓度升高,触发活动和室速或室颤发生率增加(P<0.05)。与LVH组比较,HF组LVEDd增大、LVEF降低(P<0.05),血清SOD活性降低、NT-proBNP和MDA浓度升高,触发活动和室速或室颤发生率增加(P<0.05)。结论在应激情况下,晚期HF中室速或室颤发生率明显高于LVH,这种改变与氧化应激水平增加有关。Objective To discuss the changes of oxidative stress in rabbits with myocardial hypertrophy or heart failure （HF） and explore the relations between oxidative stress and ventricular arrhythmias. Methods Forty Japanese white rabbits were randomly divided into four groups （ n = 10 each） ： the Sham 1 group （ Sham 1 ）, the Sham 2 group （ Sham 2）, the left ventricular hypertrophy（LVH） group and the heart failure（HF） group. In LVH group and HF group, the abdominal aorta was partial constricted. In Sham 1 group and Sham 2 group, the abdominal was only exposed without constriction. Rabbits in Sham 1 and LVH group were fed for 8 weeks, and those in Sham 2 and HF group were fed for 20 weeks. Left ventricular end diastolic diameter （LVEDd） ,left ventricular ejection fraction （LVEF） and interventricular septal thickness （IVST） were measured by echocardiogram. Plasma levels of N-Terminal Pro B-Type Natriuretic Peptide （NT-proBNP）, superoxide dismutase（SOD） and malondialdehyde（MAD） were measured and the wedge preparations from the rabbit hearts were perfused with Tyrode＇s solution containing isoprenaline（2 μmol/L）, and the incidences of trigger actions, ventricular tachycardias （VT） and ventricular fibrillation （VF） were recorded. Results IVST, the serum MDA was increased and the serum SOD was decreased in the LVH group compared with the Sham 1 group. The incidences of trigger actions, VT and VF were significant higher in the LVH group than those in Sham 1 group（P 〈0.05）.IVST, LVEDd, the serum MDA and NT-proBNP was increased, the serum SOD and LVEF was decreased, and the incidences of trigger actions, VT and VF were significant higher in the HF group than those in Sham 2 group （P 〈 0.05 ）. There were significant differences in MDA, NT-proBNP, SOD, LVEF and LVEDd between the LVH group and HF group （P 〈 0. 05 ）. The incidences of trigger actions, VT and VF were significant higher in the HF group than those in LVH group. Condusions The incidences of

关 键 词：心血管病学 心力衰竭 心室肥厚 氧化应激 室性心动过速 腹主动脉缩窄 

分 类 号：R541.61[医药卫生—心血管疾病] R541.7[医药卫生—内科学]