中药咳喘落对实验性哮喘豚鼠ECP和sICAM-1的影响  

Influences of KeChuanLuo on eosinophil cationic protein and soluble intercellular adhension molecule-1 in asthmatic guinea pigs

作　　者：王宏长[1] 李培成[1] 吴银根[1] 于素霞[1] 倪伟[1] 

机构地区：[1]上海中医药大学附属龙华医院呼吸内科,200032

出　　处：《广东医学》2000年第3期188-190,共3页Guangdong Medical Journal

基　　金：上海市科委重大攻关项目! (编号 9541 1 90 2 6);国家自然科学基金!资助项目 (基金编号 39670 889)

摘　　要：目的　探讨中药咳喘落对哮喘豚鼠可溶性细胞间粘附分子 -1(sICAM -1)、嗜酸性粒细胞及其脱颗粒产物嗜酸性粒细胞阳离子蛋白 (ECP)等的影响 ,阐明咳喘落治疗哮喘抗气道变应性炎症的作用机制。方法　采用荧光酶标记法、双抗体夹心ELISA法和密度梯度细胞分离法分别测定了哮喘豚鼠血浆中ECP ,sICAM -1的含量和肺泡灌洗液中嗜酸性粒细胞分类计数。结果　哮喘豚鼠血浆中ECP ,sICAM -1的含量以及肺泡灌洗液中细胞总数和低密度嗜酸性粒细胞数均显著高于正常组 (P <0 .0 1) ,经咳喘落和地塞米松治疗后显著下降 (P <0 .0 1) ,接近正常水平 (P >0 .0 5 )。结论　咳喘落通过调节白细胞介素 -5的水平 ,抑制嗜酸性粒细胞在气道内募集及其毒性颗粒蛋白的释放 ,从而发挥它抗气道变应性炎症的作用。Objective To investigate the influences of traditional Chinese herbs named KeChuanLuo on eosinophil and its degranulated substances in asthmatic guinea pigs.Methods The levels of eosinophil cationic protein (ECP) and soluble intercellular adhension molecule-1 (sICAM-1) in asthmatic guinea pigs were detected by immunolabelling technique, the number of total cells and hypodense eosinophils were simultaneously counted. Results\ The levels of ECP and sICAM-1 in serum from asthmatic guinea pigs were significantly higher than that from normal ( P <0.01), the numbers of total cells and hypodense eosinophils in bronchoalveolar lavage fluid (BALF) were also greater ( P <0.01), which was significantly reduced after KeChuanLuo or dexamethasone treatment aproaching normal value( P >0.05).Conclusion\ KeChuanLuo exerted its anti-airway allergic inflammation, by regulating interleukin-5 level and by inhibiting eosinophil recruitment and degranulation.

关 键 词：哮喘 中医药疗法 咳喘落 ECP SICAM-1 

分 类 号：R256.12[医药卫生—中医内科学] R259.622.5[医药卫生—中医学]