海洛因成瘾死亡者体内吗啡分布的免疫组化检测  

Research of Morphine Distribution of Tissues in Heroin-addicts by Immunohistochemistry

作　　者：李利华[1] 赵永和[1] 和映藻[2] 王金晖 吴亚维 李军 

机构地区：[1]昆明医学院法医学系 [2]昆明医学院成人教育学院,昆明650031 [3]昆明市公安局技术处,昆明650021 [4]昆明市公安局官渡分局,昆明650200

出　　处：《昆明医学院学报》2000年第1期85-88,共4页Journal of Kunming Medical College

基　　金：国家自然科学基金!3976 0 0 76

摘　　要：应用免疫组化技术对 6例海洛因滥用中毒死亡者体内吗啡分布进行系统研究 .研究发现 ,大脑皮质Ⅰ ,Ⅳ层神经细胞、海马锥体细胞、基底核、延髓神经细胞有强阳性吗啡分布 ,其他脑区神经细胞及部分神经纤维有弱阳性吗啡分布 .心肌、肝脏、肾脏、肾上腺、胃、肠、胰岛及注射皮肤、骨骼肌也有吗啡分布 .结果表明 :人体组织内吗啡分布与实验动物组织内吗啡分布基本一致 ,且发现脑内吗啡分布与阿片受体分布一致 .本研究说明免疫组化法可用于海洛因滥用人群的毒理病理研究及法医学鉴定 .Using immunohistochemistry technique,we researched morphine distribution of tissues in heroin-addicts with 6 cases systematically. The result showed that high positive morphine distributed in I ,IV layers' neurocytes of cerebral cortex,neurocytes of hippocampus,basal nuclei and medulla; Light positive morphine is found in other neurocytes and partial nerve fibers. Morphine is also found in myocardium,liver,kidney,adrenal gland,stomach,small intestine,islet pancreatica and injected skin,skeletal muscle. The research confirmed that morphine distribution of tissues in heroin-addicts is concordant with animal experimental results,and distribution of morphine in brain consists with μopioid receptor. From this study,we conclude that immunohistochemitry technique is a new method for toxic-pathology research and forensic medicine identification of heroin-addicts.

关 键 词：吗啡分布 免疫组织化学 海洛因依赖 

分 类 号：R595.5[医药卫生—内科学]