视黄酸对CDK_2组成性表达HL-60细胞增殖的影响  

Influence of retinoic acid on CDK_2-overexpressing HL-60 cells

作　　者：郎海滨[1] 糜漫天[1] 张乾勇[1] 韦娜[1] 黄国荣[1] 周东明[1] 

机构地区：[1]第三军医大学营养与食品卫生学教研室,重庆400038

出　　处：《免疫学杂志》2000年第2期99-101,122,共4页Immunological Journal

基　　金：国家自然基金!资助项目 (395 70 2 96 )

摘　　要：目的探讨细胞周期素依赖性激酶 2 (CDK2 )在视黄酸对急性早幼粒细胞白血病 HL- 6 0细胞的增殖抑制和分化调控过程中的作用。方法应用真核细胞基因转染技术将外源 CDK2 基因转入人 HL- 6 0早幼粒细胞白血病细胞中 ,进而以视黄酸处理 1～ 4d。结果 HL - 6 0细胞的增殖受到明显抑制 ,且出现了分化趋势 ,与对照组相比 ,更多的 CDK2 组成性表达的 HL - 6 0细胞被阻止于 G0 / G1 期 ,但与未转染的 HL - 6 0细胞相比 ,视黄酸的抑制增殖、诱导分化作用均有所削弱 ,同时 ,CDK2 的蛋白表达量变化并不明显。结论 CDK2 在视黄酸诱导的增殖抑制和分化过程中起重要作用 ,由于 CDK2 的组成性超表达 ,导致视黄酸对 HL- 6 0细胞的增殖抑制和诱导分化作用下降。Objective To determine the function and expression of CDK 2 in the course of proliferation and differentiation of HL 60 cells induced by retinoic acid(ATRA). Methods We introduced CDK 2 cDNA into HL 60 cells and treated them with ATRA. Results ATRA could inhibit the growth and induce differentiation of HL 60 cells overexpressing CDK 2, about 80 percent cells were arrested at G 0/G 1 phase after 3d exposure to ATRA Comparing with the cells without transfection, the effect of ATRA on the transfected HL 60 cells was not so much as that on the normal HL 60 cells.Moreover, the protein expression of CDK 2 did not change dramatically after exposure to ATRA. Conclusion The results above suggest CDK 2 is very important in G 1→S phase transition, it’s overexpression could promote the proliferation. ATRA could inhibit the proliferation and promote the differentiation, but CDK 2 overexpression could weaken the effect of ATRA. [

关 键 词：视黄酸 HL-60细胞 CDK2 细胞周期 白血病 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]