Survivin基因在重症急性胰腺炎并发肺损伤大鼠中的表达及作用  

Expression of Survivin gene in a rat model of severe acute pancreatitis complicated with lung injury

作　　者：李东恩[1] 樊恒[1] 宋甫春[1] 

机构地区：[1]宁波市第一医院肝胆外科,浙江宁波315020

出　　处：《肝胆胰外科杂志》2012年第6期474-476,共3页Journal of Hepatopancreatobiliary Surgery

基　　金：宁波市医学科技计划项目(2009A13)

摘　　要：目的研究大鼠重症急性胰腺炎(sever acute pancreatitis,SAP)肺损伤时凋亡抑制因子Survivin表达与肺损伤的关系。方法 40只雄性SD大鼠随机分为四组:对照组(n=10)和SAP 6 h组(n=10)、12 h组(n=10)、24 h组(n=10),采用胰胆管逆行穿刺注射4.5%牛磺胆酸钠建立SAP模型。采用RT-PCR法检测肺组织中Survivin的表达,TUNEL法检测肺组织细胞凋亡的变化。结果 SAP造模后,肺组织细胞6 h、12 h、24 h凋亡指数分别为3.97±1.67、9.69±1.01、12.10±1.71,对照组为0.171±0.045(P<0.05);Survivin蛋白mRNA的表达分别为0.512±0.169、0.813±0.201、1.431±0.251,对照组肺组织中未见Survivin蛋白mRNA表达(P<0.05)。结论 Survivin基因在SAP大鼠并发肺损伤时肺组织中呈异常高表达,Survivin表达的高低与肺组织损伤的严重程度有关,可能起到减轻肺组织损伤和促进其修复的作用。Objective To investigate the relationship between lung injury and the expression of Survivin gene in a rat model of severe acute pancreatitis. Methods Forty healthy male SD rats were randomly divided into four groups： control group （n=10） and SAP group of 6 h （n=10）, 12 h （n=10） and 24 h （n=10）. The SAP rat model was induced by injection of 4.5% sodium taurocholate into pancreatic duct. RT-PCR was applied to detect the expression of Survivin gene in the lung tissue, and the apoptosis ratio of the lung cells was determined with TUNEL assay. Results Survivin gene was highly expressed in the lung tissue of the SAP rats complicated with lung iniurv, and there was no expression in the control group. Conclusion The level of lung iniurv in SAP may concerned with the expression of Survivin, which may play a role of relieving and repairing the iniurv of lung.

关 键 词：重症急性胰腺炎 SURVIVIN 肺损伤 凋亡 大鼠 

分 类 号：R657.51[医药卫生—外科学]