绞股蓝皂甙对阿尔茨海默病小鼠海马胆碱能系统功能的影响  

Effects of gypenosides on the hippocampal cholinergic system in D-galactose induced Alzheimer's disease in mice

作　　者：周卫华[1] 谭莉明[1] 米长忠[1] 钟飞[1] 

机构地区：[1]吉首大学医学院,湖南吉首416000

出　　处：《中国老年学杂志》2012年第22期4943-4944,共2页Chinese Journal of Gerontology

基　　金：湖南省科技厅基金项目(2011TJ3202);湘西州科技局课题项目(2009zd06)

摘　　要：目的探讨绞股蓝皂甙对D-半乳糖(D-gal)所致阿尔茨海默病(AD)模型小鼠海马胆碱能系统功能的影响。方法昆明小鼠颈背部皮下注射10%D-gal,连续6 w造模。同时,各组分别灌胃生理盐水(正常对照组、模型组)、绞股蓝(高、低剂量组)。给药结束后进行水迷宫训练,24 h后进行学习记忆功能测试和海马胆碱乙酰基转移酶(ChAT)与乙酰胆碱酯酶(AchE)活性测定以及Western印迹检测ChAT表达。结果与正常对照组相比,D-gal模型组小鼠学习记忆能力,模型组ChAT活性和ChaT表达明显下降(P<0.05,P<0.01);模型组AchE活性明显升高(P<0.01)。给予绞股蓝皂甙可明显改善AD模型小鼠的学习记忆功能(P<0.05,P<0.01),增强海马ChAT活性(P<0.01)和降低AchE活性(P<0.01),上调ChAT的表达(P<0.01)。结论绞股蓝皂甙可明显提高模型小鼠的学习记忆能力,改善海马胆碱能系统功能。Objective To investigate the effects of gypenosides on the hippocampal cholinergic system in D-galactose induced Alzheimer＇s disease （AD） in mice. Methods The mice were given subcutaneous injection of 10% D-galactose for 6 weeks （ 125 ml· kg^-1·d ^- 1 ）. Normal saline, gypenosides respectively were given by intragastric administration in different study groups. The mice were trained to find the platform in the water maze on the 43th day. After 24 hours, learning and memory ability was tested, choline acetyltransferase （chAT） activity, acetylcholinesterase （AchE） activity, the expression of ChAT in hippocampus was observed by Western blotting after treatment. Results Compared with the normal control group, the learning and memory dysfunction, the decreases of ChAT activity and expression of ChAT were found in model group, AchE activity was increased in model group （P 〈 0. 05, P 〈 0. 01 ）. The gypenosides could markedly attenuate cognitive dysfunction, elevate ChAT and expression of CHAT, decrease AchE activity in the hippocampi of mice treated with D-galactose （P 〈 0.05, P 〈 0. 01 ）. Conclusions The gypenosides can significantly improve the hippocampal cholinergic system function, and thus enhance the learning and memory ability in D-galactose induced AD model mice model.

关 键 词：绞股蓝皂甙 半乳糖 阿尔茨海默病 胆碱能系统 

分 类 号：R285.5[医药卫生—中药学]