己烯雌酚对促甲状腺激素受体在小鼠淋巴结内表达的影响  

Effects of diethylstilbestrol on the expression of thyroid stimulating hormone receptor in mice lymphoid nodes

作　　者：陈莉[1] 卫兰[1] 张思宇 张京[1] 

机构地区：[1]北京大学医学部组织学与胚胎学教研室 [2]临床医学专业2007级1班,北京100191

出　　处：《解剖学报》2012年第6期796-801,共6页Acta Anatomica Sinica

基　　金：国家基础科学人才培养基金资助项目(J1030831/J0108)

摘　　要：目的了解生后不同时期BALB/c小鼠淋巴结内促甲状腺激素受体(TSHR)的表达状况及给予新生期BALB/c小鼠己烯雌酚(DES)后对该表达所产生的影响,并借此探讨生后不同时期小鼠淋巴结内促甲状腺激素(TSH)的可能来源及DES对TSH表达所产生影响的可能机制。方法选取同窝及体重差异小于5%的新生BALB/c小鼠,随机分为DES组与对照组,雌、雄各选30对,即共取小鼠120只,在生后24h内,DES组小鼠颈背部皮下注射DES,每隔24h注射1次,共连续注射5次;对照组小鼠平行注射等量无菌豆油。分别在生后第7、14、21、35、49和第63天将小鼠处死,每个时间点雌、雄各取5对小鼠,取其颈深淋巴结进行常规石蜡包埋、切片及TSHR的免疫组织化学检测。结果无论是对照组还是DES组,小鼠从生后第7天到第63天,淋巴结内均可见到TSHR阳性细胞,阳性表达在雌性和雄性小鼠分别于生后第49天和第35天达到峰值。同一时间点对照组和DES组相比较发现,无论是平均吸光度(AA)还是积分吸光度(IA),DES组均高于对照组。在雌性小鼠,两组间AA和IA在生后各时间点均有统计学差异;在雄性小鼠,两组间AA在生后第14、21、35和第49天有统计学差异,IA在第14、21、35、49和第63天有统计学差异。结论 BALB/c小鼠淋巴结细胞在生后不同时期均具有结合垂体前叶来源TSH的受体基础。新生期接触DES可导致小鼠淋巴结内TSHR阳性表达增加,雌性小鼠大于雄性小鼠。而DES对雌性和雄性小鼠淋巴结内TSHR表达的影响均可持续至小鼠成年期。Objective To investigate the expression of thyroid stimulating hormone receptor （TSHR） in lymphoid nodes of BALB/c mice and in the mice treated with diethylstilbestrol （DES） during the neonatal period, and to explore the possible sources of thyroid stimulating hormone （TSH） expressed in the lymphoid nodes and the possible mechanism of the effect of DES on the expression of TSH. Methods Neonatal BALB/c mice whose weight discrepancies were less than 5% were divided randomly into a control group and a DES group. A total of 120 mice（30 pairs of females and 30 pairs of males） were chosen. Within 24 hours after birth, the mice in the DES group were injected subcutaneously at the cervix- backside with DES at intervals of 24 hours for 5 times, and the mice in the control group were injected with germfree bean oil using the same method. Mice were killed on postnatal day 7, 14, 21, 35, 49 and 63. 5 pairs of female mice and 5 pairs of male mice at each time point. Deep cervical lymph nodes were taken out, embedded in paraffin and sliced serially in a thickness of 4p^m. The expression of TSHR in the lymphoid nodes was detected by immunohistochemistry technique. Results In both control and DES groups, TSHR positive cells were present in lymphoid nodes from postnatal day 7 to day 63. In female mice, the expression of TSHR reached peak on day 49 and in male mice on day 35. At each the same time point, the average absorbance （AA） and the integrate absorbance （M） of TSHR expression were higher in the DES group than those in the control group. In female mice, statistic differences were showed in both AA and M between the two groupsat all the time points, and in male mice statistic differences were showed in AA on day 14, 21, 35 and 49 and in IA on day 14, 21, 35, 49 and 63 between the two groups. Conclusion TSHR positive cells are present in all the lymphoid nodes of both female and male BALB/c mice at all the detected time points. This suggests that the lymphocytes in lymphoid nodes of BALB/c mice during t

关 键 词：淋巴结 己烯雌酚 促甲状腺激素受体 免疫组织化学 新生BALB C小鼠 

分 类 号：R392[医药卫生—免疫学]