骨桥蛋白在动情周期与妊娠早期小鼠输卵管上皮的表达  

Expression of osteopontin in mouse oviduct during the estrous cycle and early pregnancy

作　　者：周筠[1] 谢青贞[1] 陈颖娴[1] 

机构地区：[1]武汉大学人民医院生殖医学中心,武汉430060

出　　处：《解剖学报》2012年第6期822-825,共4页Acta Anatomica Sinica

摘　　要：目的探讨骨桥蛋白(OPN)在动情周期和妊娠早期小鼠输卵管的表达。方法 70只昆明雌性小白鼠分为动情周期组、妊娠组和假孕组,昆明雄性小白鼠15只用于与雌鼠合笼。取雌鼠动情周期、妊娠和假孕第1～5天的输卵管,利用免疫组织化学染色及半定量分析检测OPN在小鼠输卵管的定位和表达情况。结果整个动情周期小鼠输卵管上皮组织OPN的免疫染色均呈阳性,动情期的输卵管上皮OPN染色的平均吸光度值(AA)最高,动情间期组AA值最低;妊娠和假孕第1～5天小鼠输卵管上皮OPN的免疫染色均为阳性。妊娠第4天输卵管上皮OPN染色的AA值最高;妊娠组第3～5天OPN染色的AA值显著高于假孕组所对应的妊娠天数(P<0.05,P<0.01,P<0.05)。结论小鼠动情周期输卵管上皮OPN的表达可能受体内雌激素的调节,妊娠早期小鼠输卵管上皮OPN的表达可能受雌激素和着床前胚胎共同调节。Objective To investigate expression of osteopontin （OPN） in the mouse oviduct during the estrous cycle and early pregnancy. Methods A total of 70 female and 15 male mice of Kunming white strains were used in this study. Immunohistochemistry and image analysis were employed to detect and analyze the localization and expression of OPN in the mouse oviduct during the estrous cycle, pregnant and pseudopregnant from day 1 to 5, respectively. Results Throughout the estrous cycle, the OPN immunostaining of mouse oviduct epithelium was positive. The average absorbance （AA） of OPN in oviduct epithelium was the highest in the estrus stage, and the lowest in the diestrus stage. The OPN immunostaining was positive throughout day 1 to 5 of pregnancy and pseudopregnancy. The expression of OPN reached peak on day 4 of pregnancy. There were significant differences in the same day between the pregnancy and the pseudopregnancy in the AA value of OPN druing day 3 to 5 （P 〈 0.05, P 〈 0. 01, P 〈 0.05）. Conclusion The expresses of OPN in the mouse oviduct epithelium may be regulated by estrogen during the estrous cycle, which is regulated by estrogen and preimplantation embryos during early pregnancy.

关 键 词：输卵管 骨桥蛋白 动情周期 妊娠 免疫组织化学 小鼠 

分 类 号：R71[医药卫生—妇产科学]