正丁醇替代无水乙醇和二甲苯在制作大鼠全胚切片中的应用  

Replacing absolute ethanol-xylene by n-butanol during preparation of sections of whole rat embryos at different gestational ages

作　　者：杨世明[1] 李和[1] 

机构地区：[1]华中科技大学同济医学院解剖学系组织胚胎学教研室,武汉430030

出　　处：《解剖学报》2012年第6期864-867,共4页Acta Anatomica Sinica

摘　　要：目的探索一种以正丁醇替代无水乙醇和二甲苯制作不同胎龄大鼠全胚切片的简便方法。方法收集胚龄14d(E14)、E16、E18、E20大鼠胚胎,固定及低浓度乙醇脱水后,部分标本按传统方法用无水乙醇脱水和二甲苯透明(无水乙醇-二甲苯法),另一部分标本用正丁醇脱水、透明(正丁醇法)。所有标本进行石蜡包埋、切片、HE染色。对两种方法从蜡块质量和切片质量等方面进行比较和评价。结果正丁醇法大大简化了操作步骤,标本处理时间容易控制,包埋块的切割性能及对肝脏组织的保存优于无水乙醇-二甲苯制片法。结论正丁醇法步骤简单且制片安全可靠,可替代无水乙醇-二甲苯法制作大鼠全胚切片。Objective To explore a simple method to replace absolute ethanol and xylene with n-butanol for preparation of sections of whole rat embryos at different gastational ages. Methods Embryonic day （E） 14, 16, 18, 20 rat embryos were collected and fixed. After dehydration in low concentrations of the ethanol, some embryos were treated with the traditional absolute ethanol-xylene method in which absolute ethanol and xylene were used for dehydration and clearing, and other embryos were further dehydrated and cleared with the n-butanol method. Then, all the embryos were embedded in paraffin, cut on a microtome, and stained with hematoxylin-eosin. The quality of embedded blocks and embryo sections was compared and evaluated. Results Compared with the absolute ethanol-xylene method for preparation of whole rat embryo sections, the n-butanol method was more simple, and easier to control the time in the whole processes. Moreover, the embryos treated with n-butanol had better cutting performance and higher quality in morphological preservation of the hepatic tissue. Conclusion As a simple, safe and reliable method, the n-butanol method can replace the absolute ethanol- xylene method for preparation of whole rat embryo sections.

关 键 词：全胚切片 正丁醇 无水乙醇 二甲苯 苏木素-伊红染色 大鼠 

分 类 号：Q954.5[生物学—动物学]