三七总皂苷对体外培养人牙周膜细胞增殖和分化的影响  

Effects of panax notoginseng saponins on proliferation and differentiation of human periodontal ligament cells cultured in vitro

作　　者：吴静[1] 向学熔[1] 范小平[1] 

机构地区：[1]重庆医科大学附属口腔医院,重庆401147

出　　处：《中国生物制品学杂志》2012年第11期1464-1467,共4页Chinese Journal of Biologicals

基　　金：重庆市卫生局中医药科技项目(20092116)

摘　　要：目的探讨三七总皂苷对体外培养的人牙周膜细胞(Human periodontal ligament cell,hPDLC)增殖、碱性磷酸酶(Alkaline phosphatase,ALP)活性及表达的影响。方法采用组织块法原代培养hPDLC,并经免疫组化鉴定;分别用10、1.0、0.1和0.01 mg/L浓度的三七总皂苷处理体外培养的hPDLC,设只加含10%FBS的DMEM培养基为空白对照,分别于1、3、5、7 d后,MTT法检测其对hPDLC增殖活性的影响,酶标仪上检测细胞中ALP活性,Western blot法检测细胞中ALP蛋白的表达。结果免疫组化结果显示,细胞来源于外胚间充质;MTT结果显示,0.1 mg/L浓度组在第5天和1.0、10 mg/L浓度组在第3、5天hPDLC增殖活性明显高于空白对照组(P<0.01);0.1、1.0、10 mg/L浓度组在第3、5天ALP活性明显高于空白对照组(P<0.01),1.0 mg/L浓度组APL活性明显高于其他各组(P<0.01);0.01、0.1、1.0、10 mg/L浓度组ALP蛋白表达量均明显高于空白对照组(P<0.01),以1.0 mg/L浓度组ALP表达量最高。结论三七总皂苷有促进hPDLC增殖的作用,并能增加ALP活性及其表达水平。Objective To investigate the effect of panax notoginseng saponins（PNS） on proliferation,alkaline phosphatase（ALP） activity and expression of human periodontal ligament cells（hPDLCs） cultured in vitro.Methods The hPDLCs were subjected to primarily culture by tissue block method and identified by immunohistichemical assay.The hPDLCs cultured in vitro were treated with PNS at concentrations of 10,1.0,0.1 and 0.01 mg / ml respectively,using the DMEM containing 10% FBS as blank control,and determined for proliferation activity by MTT method 1,3,5 and 7 d after treatment,for ALP activity by microplate reader,and for ALP expression by Western blot.Results Immunohistichemical assay showed that hPDLCs were derived from ectomesenchyme.MTT test showed that the proliferative activities of hPDLCs on day 5 after treatment with 0.1 mg / ml and on days 3 and 5 after treatment with 1.0 and 10 mg / ml PNS were significantly higher than those in blank control group（P 0.05）.The ALP activities of hPDLCs on days 3 and 5 after treatment with 0.1,1.0 and 10 mg / L PNS were significantly higher than those in blank control group（P 0.01）,while were significantly higher after treatment with PNS at a concentration of 1.0 mg / L than at other concentrations（P 0.01）.The expression levels of ALP in hPDLCs after treatment with 0.01,0.1,1.0 and 10 mg / L PNS were significantly higher than those in blank control group（P 0.01）,of which that after treatment with 1.0 mg / L was the highest.Conclusion PNS promoted the proliferation and increased the ALP activity and expression level of hPDLCs.

关 键 词：三七总皂苷 牙周膜细胞 碱性磷酸酶 

分 类 号：R285.5[医药卫生—中药学] Q254[医药卫生—中医学]