醛化鹅红细胞的制备及其在百日咳血凝活性测定中的应用  

Preparation of hydroformylated goose red blood cells and its application in determination of hemagglutination activity of pertussis vaccine

作　　者：赵伟[1] 胡业勤[1] 薛红刚[1] 李新国[1] 刘玉兰[1] 朱红伟[1] 刘胜[1] 

机构地区：[1]武汉生物制品研究所有限责任公司细菌类疫苗室,武汉430060

出　　处：《中国生物制品学杂志》2012年第11期1535-1537,共3页Chinese Journal of Biologicals

基　　金："重大新药创制"科技重大专项(2011zx09201-301;2011zx-09401-302)

摘　　要：目的建立鹅红细胞的醛化方法,用于百日咳疫苗生产中的血凝活性测定。方法以新鲜鹅红细胞为对照,分别采用方法一、方法二醛化鹅红细胞,比较两种处理方法制备的醛化鹅红细胞的溶血性和活性,确定最佳醛化条件。结果采用方法一制备的醛化鹅红细胞的实验效果优于方法二,其测定结果与新鲜红细胞一致,在4℃保存90 d未出现溶血现象,戊二醛最佳处理浓度为1.25%,醛化时间为40 min,最佳醛化鹅红细胞工作浓度为0.8%。结论已成功建立鹅红细胞的醛化方法。Objective To develop a method for preparation of hydroformylated goose red blood cells（RBCs） and apply to determination of hemagglutination（HA） activity of pertussis vaccine.Methods Goose RBCs were hydroformylated by two methods and determined for HA titer and hemolysis,using fresh good RBCs was control,based on which the condition for hydroformylation was optimized.Results The determination results of hydroformylated goose RBCs prepared by method 1 were consist with those of fresh goose RBSs.No hemolytic reaction was observed in the hydroformylated goose RBCs prepared by method 1 after storage at 4 ℃ for 90 d.The optimal glutaraldehyde concentration and time for hydroformylation were 1.25% and 40 min respectively,while the optimal working concentration of hydroformylated goose RBCs was 0.8%.Conclusion A method for hydroformlylation of goose RBSs was successfully developed.

关 键 词：醛化红细胞 百日咳 血凝试验 

分 类 号：R378.4[医药卫生—病原生物学] R392-33[医药卫生—基础医学]