鲎血中超氧化物歧化酶对D-半乳糖致衰老小鼠抗氧化作用的研究  

Antioxidant effects of superoxide dismutase from horsefoot blood in aged mice induced by D-galactose

作　　者：卢海庆[1] 李俊[2] 刘成军[2] 韦世秀[3] 张学荣[3] 李牡艳[3] 

机构地区：[1]广西医科大学药学院 [2]广西壮族自治区江滨医院 [3]广西医科大学医学科学实验中心,广西南宁530021

出　　处：《中国药理学通报》2012年第12期1733-1736,共4页Chinese Pharmacological Bulletin

基　　金：广西科技厅科学基金资助项目(No桂科自0991116)

摘　　要：目的研究鲎血中超氧化物歧化酶(superoxide Dis-mutase,SOD)对D-半乳糖所致衰老小鼠的抗氧化作用。方法将96只昆明小鼠随机分为空白对照组、衰老模型组、维生素E组和鲎血SOD高、中、低剂量组。空白对照组颈背部注射生理盐水,其余各组颈背部注射D-半乳糖[120 mg.(kg.d)-1]造模,同时维生素E组和鲎血SOD处理组分别灌胃给药,连续造模给药42 d后各组小鼠眼球采血并取肝脏、脑组织。检测血清、肝脏、脑中的SOD、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量。结果与空白对照组相比,衰老模型组小鼠血清、肝脏、脑中SOD、GSH-Px活力明显降低(P<0.01),MDA含量明显升高(P<0.01)。与衰老模型组相比,鲎血SOD处理组在给药后均能明显提高小鼠血清中SOD和GSH-Px、肝脏中SOD、脑中GSH-Px的活力(P<0.05),降低血清和脑中MDA含量,肝脏中GSH-Px活力、MDA含量和脑中SOD活力无明显变化。维生素E组与SOD给药组效果相当。结论鲎血中SOD对D-半乳糖所致衰老模型小鼠有一定程度的抗氧化作用,对血清抗氧化作用较强,对肝脏和脑组织的效果不明显。Aim To study the antioxidant effects of superoxide dismutase（SOD） from horsefoot blood in aged mice induced by D-galactose（D-ga1）.Methods Ninety-six Kunming mice were randomly divided into no-treatment control group,aging model group,vitamin E group and SOD high-,middle-and low-doses groups.The control group mice were made by neck back subcutaneous injection of normal saline everyday.The mice in the rest of the groups were made by neck back subcutaneous injection of D-ga1[120 mg·（kg·d）-1].The mice in vitamin E group and SOD treatment groups were given by gavage respectively at the same time.42 days later,blood was sampled from eyeball of the mice in each group and liver,brain tissue were taken as well to detect the level of SOD,glutathione peroxidase（GSH-Px） and malondialdehyde（MDA） in serum,liver and brain tissue.Results Compared with control group,SOD,GSH-Px activity revealed a significant decrease（P0.01） and MAD content exhibited a significant increase（P0.01） in serum,liver and brain tissue in aging model mice.Compared with aging model group,SOD、GSH-Px activity in serum,SOD activity in liver and GSH-Px activity in brain exhibited a significant increase（P0.05） and MDA content decreased in serum and brain tissue in SOD treatment group.However GSH-Px activity in liver,SOD activity in brain and MDA content in liver had no significant changes.The antioxidant effects of vitamin E group resembled those of SOD treatment group.Conclusion SOD from horsefoot blood have a certain degree of antioxidant effects in aged mice induced by D-ga1,and the targeted effects in serum are much more significant than those in liver and brain tissue.

关 键 词：鲎血 D-半乳糖 抗氧化作用 衰老小鼠 超氧化物歧化酶 谷胱甘肽过氧化物酶 丙二醛 

分 类 号：R285.5[医药卫生—中药学]