ApoE-／-小鼠动脉粥样硬化斑块形成特点及磁共振成像的实验研究  

Atherosclerotic plaque characteristics and magnetic resonance imaging of ApoE-/- mice models

作　　者：李代欣[1] 马占龙[1] 李澄[1] 文颂 

机构地区：[1]扬州大学第二临床医学院扬州市第一人民医院影像科,江苏扬州225000 [2]江苏省分子影像与功能影像重点实验室

出　　处：《实用放射学杂志》2012年第11期1777-1780,1809,共5页Journal of Practical Radiology

基　　金：国家自然科学基金面上项目（81071193）.

摘　　要：目的建立载脂蛋白E基因敲除（ApoE-／-）小鼠颈动脉粥样硬化模型，并应用7．0TMRI对动脉粥样硬化斑块进行评价。方法通过高脂饲养以及在左侧颈总动脉外置套管的方法建立实验组ApoE-/-小鼠和对照组野生型C57小鼠颈动脉粥样硬化模型（n=6），8周后对2组小鼠行MR扫描。扫描完成后取血测定血清总胆固醇（Tc）、甘油三酯（TG）、高密度脂蛋白（HDL）和低密度脂蛋白（LDL），并取损伤侧颈动脉行HE染色观察病理学变化，并对有斑块形成的血管行OilRedO染色观察斑块内脂质成分的分布。结果实验组小鼠在建模8周后MRI表现为损伤侧血管壁信号增高、管腔不规则狭窄，相应的组织病理学检查显示损伤侧动脉管腔狭窄，其内可见粥样斑块形成；而对照组小鼠在建模8周后MRI表现仅见2例血管壁信号增高、管腔狭窄，相应的组织病理学检查显示损伤侧动脉管壁增厚，未见粥样斑块形成。实验组小鼠血清TC和LDL值明显升高（t值分别为9．99和9．12，P值均〈0．01），TG和HDI。值无明显变化（t值分别为-1．71和-1．05，P值〉0．05）。结论高脂饲养结合颈总动脉外置套管的方法可以快速建立ApoE-/-小鼠动脉粥样硬化模型，应用7．0TMRI可成功对动脉粥样硬化斑块进行评价。Objective To establish the ApoE / carotid atherosclerosis mice model and to visualize the atherosclerotic plaque by 7.0T MR scanner. Methods High fat diet were fed and silastic collars were placed around the left carotid artery of ApoE-/- mice and wild type C57 mice to establish carotid atherosclerosis model（n= 6）. The atherosclerotic plaque formation in the left carotid were observed by MRI after eight weeks, and blood samples were taken to measure serum total cholesterol （TC） ,triglyceride {TG） , high-density lipoprotein （HDL） and low-density lipoprotein {LDL） and the left carotid artery specimens were taken to stain with HE to observe the pathological changes,and the plaques were taken to stain with Oil Red O to observe the fat. Results The vessel wall of the left carotid showed hyperintensity and the lumen were irregularly narrowed on MRI in experimental group after 8 weeks. And the histopathological examination showed atherosclerotic plaque in the vessel wall of the left carotid and the lumens were irregularly narrow. In the control group, only two cases showed hyperintensity in the vessel wall of the left carotid and irregularly narrow lumens by MRI,and the histopathological examination showed thickened arterial wall with no atheroselerotic plaque formation in the lumen 10f carotid. TC and LDL levels of experimental group were significantly higher than those of control group （t=9.99 and 9.12, respectively,P〈0.01} ,and the TG and HDL values showed no significant between-group difference （t=-1.71 and -1.05,P〉0.05 ）. Conclusion Carotid artery atherosclerosis of ApoE /- mice can be quickly established through high fat diet and placing carotid collar, and 7.0T MR] can successfully evaluate the carotid atherosclerotic plaque.

关 键 词：动脉粥样硬化 磁共振成像 小鼠 模型 

分 类 号：R541.4[医药卫生—心血管疾病] R445.2[医药卫生—内科学]