人内皮细胞和4种细胞因子对正常人和再生障碍性贫血患者粒系造血的影响  

EFFECTS OF HUMAN ENDOTHELIAL CELLS AND FOUR CYTOKINES ON GRANULOPOIESIS OF NORMAL SUBJECTS AND PATIENTS WITH APLASTIC ANEMIA

作　　者：张锑[1] 徐从高[1] 刘海鹏 张春青[1] 张茂宏[1] 

机构地区：[1]山东医科大学血液学研究室

出　　处：《山东医科大学学报》2000年第1期84-87,共4页Acta Academiae Medicinae Shandong

摘　　要：目的 :通过人脐静脉内皮细胞为铺层的双层细胞培养 ,观察内皮细胞和几种细胞因子对正常人和再障患者粒系造血的影响。方法 :以人内皮细胞作铺层 ,作体外骨髓 CFU- GM半固体双层培养 ,并在培养体系中加入细胞因子 GM- CSF、IL- 2、IL- 6和 LAK细胞上清 ,观察内皮细胞和细胞因子对正常人和再障患者粒系造血的影响。结果 :在有内皮细胞的培养体系中 CFU- GM的产率均显著高于无内皮细胞的相应培养体系。在无内皮细胞时 ,IL- 6和 LAK上清显示出与 GM- CSF相似的粒系造血刺激作用。对 1 5例初诊慢性再障患者骨髓粒系祖细胞培养结果表明 ,无内皮细胞存在时 ,无论向培养体系中加入何种细胞因子 ,均无集落生成 ,而在有内皮细胞的双层培养中 ,部分患者骨髓可见 CFU- GM集落生成。结论 :内皮细胞对正常和再障粒系造血均有明显影响 ,并提示再障造血障碍有微环境因素。Objective: The present study investigated the effects of endothelial cells and some cytokines in vitro on granulopoiesis of normal subjects and patients with aplastic anemia, with a double layer culture technique using endothelial cells derived from human umbilical cord as underlying feeding layer. Methods: Double layer culture technique with endothelial cells derived form human umbilical cord as underlying feeding layer was used to study the effects of endothelial cells and GM CFS,IL 2,IL 6, and CD 3 LAK supernant on in vitro granulopoiesis of normal subjects and patients with aplastic anemia. Results: In the presence of endothelial cells the yield of CFU GM was significantly higher than that of controls. In the absence of endothelial cells IL 6 and CD 3 LAK supernatant demonstrated a similar stimulating action on granulopoiesis as compared to GM CSF. No growth of CFU GM was a uniform characteristic in semi solid culture without endothelial cells in patients with aplastic anemia despite the addition of any cytokine investigated. On the contrary, granulocyte macrophage colonies did form in some cultures of patients with aplastic anemia in the presence of endothelial cells. Conclusion: Endothelium as one of the cellular factors of hematopoietic microenvironment supports granulopoiesis and also implicates certain defects of the microenvironment in patients with aplastic anemia.

关 键 词：内皮细胞 细胞因子 再生障碍性贫血 造血干细胞 

分 类 号：R556.5[医药卫生—血液循环系统疾病]