无标记纳米金传感显色测定五氯苯酚  

Label-free colorimetric sensing detection of pentachlorophenol with Au nanoparticles

作　　者：姚英华 李程程[2] 蔡青云[2] 

机构地区：[1]湖南益阳龙州中学,湖南益阳431000 [2]湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《化学传感器》2012年第3期38-42,共5页Chemical Sensors

基　　金：国家973项目支持

摘　　要：该文基于纳米金(AuNPs)颜色随盐致聚集而变化的特点,发展了测定五氯苯酚的无标记纳米金传感显色法。金纳米粒子在一定浓度盐的作用下发生团聚,而单链DNA(ssDNA)可以吸附在金纳米粒子表面保护金纳米粒子不团聚。羟基自由基能破坏ssDNA的结构,使其丧失对金纳米粒子的保护功能。该文利用PCP可以消耗羟基自由基,保护ssDNA,从而保护金纳米溶胶的稳定性,实现对PCP的测定。The label-free colorimetric sensing detection of pentachlorophenol （PCP） is proposed based on the salt- induced aggregation of Au nanoparticles （NPs） which results in the change in color of Au NPs. The Au NPs can aggregate in the presence of salt like NaC1, and ssDNA can protect the Au NPs from the salt-induced aggregation. Hydroxyl radical （. OH） can damage ssDNA leading to the loss of its protection ability, while PCP was found to consume the ~ OH, and consequently protect the ssDNA as well as the Au NPs from the salt-induced aggregation. The analysis of PCP is therefore proposed based on this reactions. A wide-range linearity of 1 nmol/L to 1 mmol/L is achieved with a limit of detection of 0.5 nmol/L.

关 键 词：纳米金 五氯苯酚 比色测定 传感器 持久性有机污染物 

分 类 号：X832[环境科学与工程—环境工程] O657.3[理学—分析化学]