脂膜微囊向脑损伤区定向聚集的实验研究  

The aggregation of lipid-coated microbubbles for a radi ofrequency lesion in the rat brain

作　　者：李新钢[1] 李良民[2] 郭媛[1] 何士月 李刚[1] 张庆林[1] 鲍修风[1] 吴承远[1] 

机构地区：[1]山东医科大学附属医院神经外科,济南250012 [2]山东郓城县人民医院脑外科 [3]美国康州大学医院卫生中心神经外科

出　　处：《中华神经外科杂志》2000年第2期97-100,共4页Chinese Journal of Neurosurgery

基　　金：山东省自然科学基金

摘　　要：目的　观察脂膜微囊向鼠脑损伤区聚集的趋向性 ;研究其时相分布及区域分布规律 ;探讨其作用机理。方法　建立射频脑损伤动物模型 ,静脉注射脂膜微囊混悬液 ,荧光素及油红 O染色 ,观察并计数脑切片中脂膜微囊 ,对切片进行双重免疫组化染色 ,计数增生细胞核抗原 (PCNA)和胶原纤维酸性蛋白 (GFAP)阳性细胞。结果　发现脂膜微囊聚集在脑损伤区周围 2mm的范围内 ,伤后 10天出现密度高峰 ,而PCNA、GFAP表达阳性细胞在伤后 48小时密度达高峰。脂膜微囊在伤后第 1日的聚集数量分别为PCNA和GFAP高峰数量的 42倍和 2 0倍。结论　脂膜微囊对鼠脑损伤区有特殊的亲和力 ,能定向聚集于损伤区周围 ,其机理尚需进一步研究。Objective To investigate the affinity of lipid coated microbubbles for an injured site in rat brain and its time distribution as well as region distribution in the lesion. Methods After establishing animal model of radiofrequency brain lesion and intravenous administrating the lipid coated microbubbles(LCM). The Oil O stain for LCM,immunohistochemistry for expression of proliferating cell nuclear antigen(PCNA) and glial fibrillary acidic protein (GFAP) were applied for rat brain sections. Results The aggregation of LCM was detected around the necrosis lesion within 2 mm of range and the peak density was at 10th day postinjury. The peak density of PCNA, GFAP expressing cell was at 48 h after injury. Conclusions The LCM can aggregate in rat brain injury site and has a special affinity with brain injury area. Moreover, the mechanism about this affinity need further to be investgated.

关 键 词：脂膜微囊 脑损伤 射频损伤 实验研究 

分 类 号：R944[医药卫生—药剂学]