PDGF在低氧内皮细胞条件培养基促猪肺动脉平滑肌细胞增殖中的作用  

The Effect of Platelet-derived Growth Factor in Hypoxic Endothelial Cells Conditioned Medium on the Pulmonary Artery Smooth Muscle Cell Proliferation

作　　者：廖国宁[1] 陈蓓蓓[1] 雷景迈[1] 何善述[1] 

机构地区：[1]同济医科大学基础医学院生物化学教研室,武汉430030

出　　处：《同济医科大学学报》2000年第2期105-107,共3页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金!资助项目 (No. 392 70 2 95 )

摘　　要：为了探索低氧条件下猪肺动脉内皮细胞 (PAEC)对猪肺动脉平滑肌细胞 (PASMC)增殖调控关系 ,应用MTT比色法 ,DNA荧光测定法观察了外源性 PDGF促 PASMC增殖作用 ,以及 Suram in抑制 PDGF促增殖作用的效应 ,并在此基础上研究了猪肺动脉内皮细胞条件培养基 (HECCM)中 PDGF促 PASMC增殖的作用。结果表明 ,HECCM促 PASMC增殖作用的效应可被 Suramin部分抑制 ,由此证明 ,PDGF是低氧刺激 PAEC产生的一种促 PASMC增殖的重要因子。In order to study the effect of platelet derived growth factor (PDGF) in hypoxic endothelial cells conditioned medium (HECCM) on the pulmonary artery smooth muscle cell (PASMC) proliferation, MTT colorimetry and DNA fluoremetry were used to observe the enhancement of PASMC proliferation by exogenous PEGF and the inhibitory effects of Suramin on the enhancement of PDGF. Based on the above findings, the promotion of PDGF in HECCM on PASMC proliferation was studied. The results showed that PDGF in HECCM could promote the proliferation of PASMC, which could be partly inhibited by Suramin. It was suggested that PDGF, produced by pulmonary artery endotherial cells through hypoxic stimulation, might be an important factor promoting PASMC proliferation.

关 键 词：低氧 肺动脉高压 PDGF 细胞增殖 PAEC 

分 类 号：R543.202[医药卫生—心血管疾病]