重组霍乱毒素B亚基与恶性疟原虫多表位融合蛋白的免疫原性研究  

Immunogenicity of recombinant fusion protein consisting of cholera toxin B subunit and multi valent Plasmodium falciparum epitopes

作　　者：李平[1] 曹诚[1] 钟辉[1] 石成华[1] 马清钧[1] 

机构地区：[1]军事医学科学院生物工程研究所

出　　处：《中华微生物学和免疫学杂志》2000年第2期149-151,共3页Chinese Journal of Microbiology and Immunology

摘　　要：目的　评价霍乱毒素B亚基与恶性疟原虫多价抗原的融合蛋白的免疫原性。方法　在大肠杆菌中表达重组霍乱毒素与恶性疟原虫多价抗原的融合蛋白 ,通过免疫小鼠评价融合蛋白的免疫原性。结果　通过亲和层析纯化霍乱毒素B亚基与恶性疟原虫多价抗原的融合蛋白 ,不加任何佐剂 ,以 5 0 μg/只的剂量免疫C5 7BL/ 6J小鼠 ,3次免疫后 ,CTB抗体滴度达 1∶6 40 0 ,抗疟原虫抗体滴度 1∶16 0 0 ,其CTL活性为 2 0 7%。结论　霍乱毒素具有较好的佐剂作用 ,经融合蛋白免疫的小鼠能产生良好的体液和细胞免疫 。Objective To evaluate the immunogenicity of the recombinant fusion protein containing cholera toxin B and multi valent Plasmodium falciparum protective epitopes. Methods Fusion protein was expressed in E.coli, and the immunogenicity was evaluated by inoculating C57BL/6J mice with the recombinant protein. Results The fusion protein was purified with affinity chromatography after fermentation, high titer of anti CTB antibody (1∶6?400) and anti malaria antibody (1∶1?600) were detected after the mice were vaccinated without any adjuvant, and high level of CTL activity(20.7%) was also showed. Conclusion The fusion protein retains the antigenicity and immunogenicity of cholera toxin B subunit and the malaria epitopes and is a potential vaccine against malaria.

关 键 词：霍乱霉素 疟疾 疫苗 免疫原性 恶性疟原虫 

分 类 号：R392[医药卫生—免疫学]