均匀设计法优化马缨丹提取工艺研究  

Uniform design method for optimizing the extraction technology of Lantana Camara

作　　者：尹永芹[1] 严优芍[1] 崔红花[1] 沈志滨[1] 谢浚明[1] 

机构地区：[1]广东药学院中药学院,广东广州510006

出　　处：《广东药学院学报》2012年第5期506-509,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金项目(81202885);科技部"十二五"重大新药创制项目(2011ZX09102-007-03);广东省科技厅科技计划重点专项(2011A030100013);广东省科技厅科技计划项目(2010B030700044);2010年广东省教育厅高层次人才资助项目

摘　　要：目的优选马缨丹总黄酮的最佳工艺条件。方法以马缨丹中总黄酮的含量为评价指标,采用均匀设计法优选最佳工艺条件。以索氏加热回流提取法提取,芦丁为对照品,采用紫外-可见分光光度仪在406 nm波长处测定吸光度。结果马缨丹总黄酮最佳提取工艺条件为:乙醇体积分数90%,液料比60∶1,药材粉碎度24目,提取时间4 h。结论所优选的工艺可行、重复性好。Objective To optimize the extraction process of flavonoids from Lantana camara.Methods The total flavonoids from L.camara were determined to obtain the best conditions by uniform design test,using soxhlet extraction method with rutin as reference at 406 nm wavelength.Results The uniform design experiments resulted the optimal process as follows： the extraction with 90% ethanol for 4 hours,the solid-liquid ratio of 1∶60,and particle degree of 24 mesh by soxhlet extractor.Conclusion The optimized technology is feasible with good repeatability.

关 键 词：马缨丹 总黄酮 均匀设计法 工艺优化 

分 类 号：R284.2[医药卫生—中药学]