生津益气口服液的质量标准的建立  

Quality Standard of Shengjin Yiqi Oral Liquids

作　　者：刁保忠[1] 王登旭 石磊[1] 连丽[1] 

机构地区：[1]聊城市人民医院,山东聊城252000 [2]聊城市药品检验所

出　　处：《中国药师》2012年第10期1418-1420,共3页China Pharmacist

摘　　要：目的:建立生津益气口服液的质量标准。方法:采用薄层色谱法对生津益气口服液合剂中的黄芪、五味子、黄连、赤芍进行鉴别,采用HPLC-ELSD法测定黄芪甲苷的含量,色谱柱为Agilent C18柱,流动相为乙腈-水(32∶68),流速为1.0 ml·min-1,进样量为10μL,柱温为35℃,ELSD检测器漂移管温度为100℃,载气流量为2.7 L.min-1。结果:在TLC色谱中黄芪、五味子、黄连、赤芍均能检出;黄芪甲苷在0.11～0.90 mg·ml-1范围内呈良好的线性关系,r=0.999 3。平均加样回收率为97.3%,RSD为1.13%(n=6)。结论:该法简便,准确,专属性强,能有效控制生津益气口服液的质量。Objective： To establish the quality standard for Shengjin Yiqi oral liquids.Method： Astragali Radix,Schisandrae Chinensis Fructus,Coptidis Rhizoma and Paeohiae Radix Rubra were identified by TLC,and the content of astragaloside IV was determined by HPLC-ELSD.Result： Astragali Radix,Schisandrae Chinensis Fructus,Coptidis Rhizoma and Paeohiae Radix Rubra were all detected by TLC.Astragaloside IV showed a good linear relationship within the range of 0.11-0.90 mg·ml-1（r=0.999 3）.The average recovery was 97.3% and RSD was 1.13%（n=6）.Conclusion： The method is simple and accurate with good specificity,and can control the quality of Shengjin Yiqi oral liquids effectively.

关 键 词：生津益气口服液 黄芪甲苷 薄层色谱法 高效液相色谱法-蒸发光散射检测器 

分 类 号：TQ460.72[医药卫生—药物分析学]