Expression of the B-Cell Lymphoma/Leukemia 11A Gene in Malignant Hematological Cell Lines through Quantitative Reverse Transcription Polymerase Chain Reaction  

作　　者：Yang-jun GAO Don-g-mei HE Shao-hua CHEN Xiao-juan YAN Xiao-mao HU Yang-qiu LP 

机构地区：[1]Institute of Hematology, Medical College,Jinan University, Guangzhou 510632,Guangdong Province, China [2]Key Laboratory for Regenerative Medicineof Ministry of Education, Jinan University,Guangzhou 510632, Guangdong Province,China

出　　处：《Clinical oncology and cancer researeh》2011年第4期242-246,共5页

摘　　要：The B-cell lymphoma/leukemia 11A （BCL11A） gene is essential for normal lymphoid development and has been associated with hematological malignancies. In the current study, the relative expression level of BCL11A in malignant hematological cell lines was evaluated through real-time quantitative reverse transcription polymerase chain reaction （qRT-PCR）. METHODS The relative expression level of BCLllA mRNA in malignant hematological cell lines was determined through qRT- PCR using SYBR Green I dye. Glyceraldehyde-3-phosphate dehydro- genase was used as the reference gene to confirm the relative expression level of BCL11A gene mRNA. RESULTS The relative expression level of BCL11A mRNA in cell lines from B-cell malignancies was significantly higher compared with that from acute rnyeloid leukemia （P 〈 0.05）. Different cell lines with malignant B-cells exhibited a wide range of BCL11A expressions ranging from 27.37 to 93.38. CONCLUSION The overexpression of BCL11A gene mRNA in malignant B-cells might play a role in B-cell lymphoma/leukemia.

关 键 词：B-cell lymphoma/leukemia 11A （BCL11A） malignantB-cells real-time quantitative reverse transcription-polymerasechain reaction. 

分 类 号：Q78[生物学—分子生物学] S858.317.4[农业科学—临床兽医学]