Comparison on In-Vitro Culture Methods of Yak Endometrial Gland Epithelial Cells  

作　　者：WU Qing-xia DONG Hal-long RUI Ya-pei 

机构地区：[1]College of Animal Science, Tibet Agricultural and Animal Husbandry College, Linzhi 860000, China

出　　处：《Animal Husbandry and Feed Science》2011年第4期9-12,共4页动物与饲料科学（英文版）

基　　金：supported by the Key Project of Chinese Ministry of Education (210216);the Third Phase Construction Fee for High-Level Personnel of 211 Project (SZRC-211-05)

摘　　要：[ Objective] To develop in-vitro culture methods of yak endometrial gland epithelial cells. [Method] The gland epithelial cells were isolated from yak endometrium by explant culture method and digestion culture method, respectively. [ Result] In the first method, the cells isolated from the endometrium explant could merge into monolayer after 8-d culture, and they could be purified by gradation digestion with trypsin. In the second method, the endometrium explant were first digested by collagenase II by incubation at 37℃ for 2.5 h and then further digested in fresh 2 g/L colla- genase II for another 2.5 h. The cell suspension was leached through 74 pm filter and centrifuged at 400 r/min for 5 min. Then the cell pellet was re-suspended, followed by natural sedimentation to collect purified gland epithelial cells. The isolated cells were cytokeratin-positive as detected by immunocytochemical staining, and the positive rate could reach 95%. [Conclusion] The yak endometrial gland epithelial cells can be isolated and purified by both the explant culture method and digestion culture method.[ Objective] To develop in-vitro culture methods of yak endometrial gland epithelial cells. [Method] The gland epithelial cells were isolated from yak endometrium by explant culture method and digestion culture method, respectively. [ Result] In the first method, the cells isolated from the endometrium explant could merge into monolayer after 8-d culture, and they could be purified by gradation digestion with trypsin. In the second method, the endometrium explant were first digested by collagenase II by incubation at 37℃ for 2.5 h and then further digested in fresh 2 g/L colla- genase II for another 2.5 h. The cell suspension was leached through 74 pm filter and centrifuged at 400 r/min for 5 min. Then the cell pellet was re-suspended, followed by natural sedimentation to collect purified gland epithelial cells. The isolated cells were cytokeratin-positive as detected by immunocytochemical staining, and the positive rate could reach 95%. [Conclusion] The yak endometrial gland epithelial cells can be isolated and purified by both the explant culture method and digestion culture method.

关 键 词：YAK ENDOMETRIUM Gland epithelial cells In-vitro culture 

分 类 号：S813.3[农业科学—畜牧学] S829.1[农业科学—畜牧兽医]