Isolation and Culture of Fetal Fibroblasts of Tibetan Yellow Cattle  

作　　者：CHAMBA Yang-zom 

机构地区：[1]Faculty of Animal Science,Tibet Agricultural and Animal Husbandry College

出　　处：《Animal Husbandry and Feed Science》2011年第5期3-5,8,共4页动物与饲料科学（英文版）

基　　金：supported by National Natural Science Foundation of China (31040077);University Teachers Team Construction Program of"211 Project"(SZRC-211-01);Regional Natural Science Foundation of Tibet Science and Technology Department (2010-17-02)

摘　　要：[ Objective] To isolate fetal fibroblasts from Tibetan yellow cattle and thus to provide the ideal biological material for cloning and cellular biology of animals inhabiting in the Tibet Plateau. [ Method] The fibroblast cells were isolated from tissues of Tibetan yellow fetus by explant culture method. The subculture, morphology and dynamics were observed. [ Result] The fetal fibroblasts of Tibetan yellow cattle had typical morphology of fibroblast calls. The isolated cells had three growth stages including retention period （0 -2 d）, logarithmic phase （2 -7 d） and platform phase （7 -8 d）. Its doubling time was 60 h. [ Conclusion] Fetal fibroblasts of Tibetan yellow cattle have been successfully cultured.[ Objective] To isolate fetal fibroblasts from Tibetan yellow cattle and thus to provide the ideal biological material for cloning and cellular biology of animals inhabiting in the Tibet Plateau. [ Method] The fibroblast cells were isolated from tissues of Tibetan yellow fetus by explant culture method. The subculture, morphology and dynamics were observed. [ Result] The fetal fibroblasts of Tibetan yellow cattle had typical morphology of fibroblast calls. The isolated cells had three growth stages including retention period （0 -2 d）, logarithmic phase （2 -7 d） and platform phase （7 -8 d）. Its doubling time was 60 h. [ Conclusion] Fetal fibroblasts of Tibetan yellow cattle have been successfully cultured.

关 键 词：Tibet yellow cattle Fetal fibroblasts In vitro culture ISOLATION 

分 类 号：S823[农业科学—畜牧学]