Improvement of Polyacrylamide Gel Electrophorus in Phytophthora infeatans SSR Marker  

聚丙烯酰胺凝胶电泳检测致病疫霉菌SSR标记方法改良(英文)

作　　者：张芳[1] 权军利[1] 单卫星[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《Plant Diseases and Pests》2011年第4期3-5,共3页植物病虫害研究（英文版）

基　　金：Supported by Special Research Project of National Nonprofit Industry(3-20);Funded Projects of Modern Agricultural Technology System(nycytx-15)~~

摘　　要：[Objective] The paper was to improve polyacrylamide gel electrophorus in Phytophthora infeatans SSR Marker.[Method] With the disease sample of P.infeatans collected from Guyuan in Ningxia Province in 2009 as test material,its DNA was extracted and amplified with PCR,and its products were carried out polyacrylamide gel electrophoresis.[Result] 12% polyacrylamide gel electrophoresis was used to detect primers D13,G11 and PI02,and 8% polyacrylamide gel electrophoresis was used to detect primers PI4B,PI63,SSR4,SSR8 and SSR11,then 0.1% silver nitrate was used to stain,and an ideal electrophoresis and staining effect was obtained.[Conclusion] The electrophoresis and staining method suitable for P.infeatans SSR Marker established in the study had the characteristics of high sensitivity,simple operation and clear bands,which was an effective,simple and quick detection method.[Objective] The paper was to improve polyacrylamide gel electrophorus in Phytophthora infeatans SSR Marker.[Method] With the disease sample of P.infeatans collected from Guyuan in Ningxia Province in 2009 as test material,its DNA was extracted and amplified with PCR,and its products were carried out polyacrylamide gel electrophoresis.[Result] 12% polyacrylamide gel electrophoresis was used to detect primers D13,G11 and PI02,and 8% polyacrylamide gel electrophoresis was used to detect primers PI4B,PI63,SSR4,SSR8 and SSR11,then 0.1% silver nitrate was used to stain,and an ideal electrophoresis and staining effect was obtained.[Conclusion] The electrophoresis and staining method suitable for P.infeatans SSR Marker established in the study had the characteristics of high sensitivity,simple operation and clear bands,which was an effective,simple and quick detection method.

关 键 词：Polyacrylamide gel electrophorus Phytophthora infestans SSR China 

分 类 号：S432.4[农业科学—植物病理学]