Influence of 6-BA on Incidence Rate of Chinese Cabbage Clubroot (Plasmodiophora brassicae Woron)  

6-BA对大白菜根肿病发病率的影响(英文)

作　　者：王哲[1] 郑萍 刘照斌[3] 马有会[1] 王火旭[1,4] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116081 [2]临西县技工学校,河北邢台054900 [3]辽宁师范大学分析测试中心,辽宁大连116029 [4]辽宁师范大学生命科学学院辽宁省生物技术重点实验室,辽宁大连116081

出　　处：《Plant Diseases and Pests》2011年第5期21-24,共4页植物病虫害研究（英文版）

摘　　要：[Objective] The paper was to study the relationship between cytokinin and Chinese cabbage clubroot, and to explore the incidence mechanism of clubroot. [Method]The spores were extracted from the tumors of diseased plant, and their DNA was extracted for PCR test. The diagnosed spores were inoculated to the culture soils in erlenmeyer flask at seeding stage, at seed germination and 21 d after sowing, and 0.08 μmol/L 6-BA was added to the soil at germination. Spores were extracted from the root tumors developed in inoculated plants and examined by scanning electronic microscope （ SEM）, and the incidence rate of clubroot in 6-BA treatment and control was recorded. [ Result] Plasmodiophora brassicae Woron could cause the clubroot incidence of Chinese cabbage planted in erlenmeyer flasks, the incidence rate of chibroot in the treatment adding with 0.08 μmol/L 6-BA was 100%, while that in the treatment without 6-BA was 57%, and the volume of former tumor was much larger than the latter. SEM showed that the size of resting spore of P. brassicae was 1.5 -4.3μm. [ Conduslon] 6-BA applied at germination could significantly promote the formation of tumor of Chinese cabbage clubroot.[Objective] The paper was to study the relationship between cytokinin and Chinese cabbage clubroot, and to explore the incidence mechanism of clubroot. [Method]The spores were extracted from the tumors of diseased plant, and their DNA was extracted for PCR test. The diagnosed spores were inoculated to the culture soils in erlenmeyer flask at seeding stage, at seed germination and 21 d after sowing, and 0.08 μmol/L 6-BA was added to the soil at germination. Spores were extracted from the root tumors developed in inoculated plants and examined by scanning electronic microscope （ SEM）, and the incidence rate of clubroot in 6-BA treatment and control was recorded. [ Result] Plasmodiophora brassicae Woron could cause the clubroot incidence of Chinese cabbage planted in erlenmeyer flasks, the incidence rate of chibroot in the treatment adding with 0.08 μmol/L 6-BA was 100%, while that in the treatment without 6-BA was 57%, and the volume of former tumor was much larger than the latter. SEM showed that the size of resting spore of P. brassicae was 1.5 -4.3μm. [ Conduslon] 6-BA applied at germination could significantly promote the formation of tumor of Chinese cabbage clubroot.

关 键 词：6-BA Chinese cabbage CLUBROOT Scanning electronic microscope PCR technology China 

分 类 号：S436.341[农业科学—农业昆虫与害虫防治]