Identification and Susceptibility Test of Ralstonia solanacearum Isolated from White Burley  

白肋烟青枯菌的分离鉴定及药敏性研究(英文)

作　　者：李洪[1] 严占勇[2] 杨水英[3] 吴卫玲[1] 王晗[1] 李振轮[1] 

机构地区：[1]西南大学资源环境学院,重庆400716 [2]四川省烟草公司达州市公司,四川达州635006 [3]西南大学植物保护学院,重庆400716

出　　处：《Plant Diseases and Pests》2011年第5期61-64,共4页植物病虫害研究（英文版）

基　　金：Supported by Sichuan Tobacco Monopoly Bureau Project ( 200901009)~~

摘　　要：[ Objective ] The paper was to isolate and identify Ralstonia solanacearum from white burley, and determine its susceptibility to 6 fungicides. [ Mcth- od] Using the combination method of semiselective medium （PCCG） and apolymerase chain reaction （PCR） technique, R. solanacearum in stalk of white burley from Dazhou City in Sichnan Province was isolated, and its biochemical type was identified. Through susceptibility test, the susceptibility of R. solanacearum to bismerthiazol, ethylicin, streptomycin, lime sulfur, 47% polylysine, 99% kojic acid was studied in laboratory. [Result] A total of 23 strains OfR. solanacearum were isolated, all belonging to biochemical type Ill. R. solanacearum obtained in the test were more susceptible to ethylicin, streptomycin and bismerthiazol, and ethylicin had good control effect against R. solanacearum with ECso of 0.086 ml/L. [ Conclusion ] The study provide theoretical basis for control of R. solanaceanon in white burley.[ Objective ] The paper was to isolate and identify Ralstonia solanacearum from white burley, and determine its susceptibility to 6 fungicides. [ Mcth- od] Using the combination method of semiselective medium （PCCG） and apolymerase chain reaction （PCR） technique, R. solanacearum in stalk of white burley from Dazhou City in Sichnan Province was isolated, and its biochemical type was identified. Through susceptibility test, the susceptibility of R. solanacearum to bismerthiazol, ethylicin, streptomycin, lime sulfur, 47% polylysine, 99% kojic acid was studied in laboratory. [Result] A total of 23 strains OfR. solanacearum were isolated, all belonging to biochemical type Ill. R. solanacearum obtained in the test were more susceptible to ethylicin, streptomycin and bismerthiazol, and ethylicin had good control effect against R. solanacearum with ECso of 0.086 ml/L. [ Conclusion ] The study provide theoretical basis for control of R. solanaceanon in white burley.

关 键 词：Ralstonia solanacearum Bacteria isolation IDENTIFICATION PCCG Susceptibility test China 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]