Phagocytosis Conditions of Peripheral Blood Leucocytes in Chicken  

作　　者：QIN Feng YU Sheng-lan CHEN Yu-yong FANG Xi-xiu YU Jie 

机构地区：[1]Jiangsu Province Veterinary Bio-pharmaceutical High-tech Key Laboratory, Taizhou 225300, China [2]Department of Food Science and Technology, Jiangsu Animal Husbandry & Veterinary College, Taizhou 225300, China [3]Institute of Nutrition and Feed, Jiangsu Animal Husbandry & Veterinary College, Taizhou 225300, China

出　　处：《Animal Husbandry and Feed Science》2010年第1期14-16,共3页动物与饲料科学（英文版）

基　　金：funding from the Jiangsu Province Veterinary bio-pharmaceutical high-tech Key Laboratory Foundation;Jiangsu Taizhou Scientific and Technological Project ( TL0719)

摘　　要：[Objective] To investigate the optimal conditions of peripheral blood leucocytes phagocytosing Staphy/ococcus spp. [Method] With Staphylococcus spp. 10 145 as tested materials, phagocytosis activity of chicken＇s peripheral blood leucocytes was detected by the method of Wright＇s staining. The effects of time, temperature and concentration of Staphylococcus spp. 10 145 on phagocytosis percentages （PP） and phagocytosis index （PI） were optimized by orthogonal test and their morphologic characteristics were observed by microscope. [ Result] The concentration of Staphylococcus spp. 10 145 was the primary factor effecting leucocytes phagocytosis index. The optimal phagocytosis conditions were at the temperature of 41℃, time of 40 min and concentration of Staphylococcus spp. 10 145 of 5 ×10^ 6/ml. The order of various factors on leucocytes phagocytcsis index was the concentration of Staphylococcus spp. 10 145 〉 time 〉 temperature. [ Conclusion] This study provides a more simple and reliable method for detecting leucocytes phagocytosis function.[Objective] To investigate the optimal conditions of peripheral blood leucocytes phagocytosing Staphy/ococcus spp. [Method] With Staphylococcus spp. 10 145 as tested materials, phagocytosis activity of chicken＇s peripheral blood leucocytes was detected by the method of Wright＇s staining. The effects of time, temperature and concentration of Staphylococcus spp. 10 145 on phagocytosis percentages （PP） and phagocytosis index （PI） were optimized by orthogonal test and their morphologic characteristics were observed by microscope. [ Result] The concentration of Staphylococcus spp. 10 145 was the primary factor effecting leucocytes phagocytosis index. The optimal phagocytosis conditions were at the temperature of 41℃, time of 40 min and concentration of Staphylococcus spp. 10 145 of 5 ×10^ 6/ml. The order of various factors on leucocytes phagocytcsis index was the concentration of Staphylococcus spp. 10 145 〉 time 〉 temperature. [ Conclusion] This study provides a more simple and reliable method for detecting leucocytes phagocytosis function.

关 键 词：LEUCOCYTES Phagocytose Staphylococcus spp. 

分 类 号：S965.116[农业科学—水产养殖] Q939.91[农业科学—水产科学]