Discrimination of Goatpoxvirus and Sheeppoxvirus by Digestion of p32 Gene with Hinf I and Sequence Alignment of GpCR Gene  

作　　者：YAN Xin-min ZHANG Qiang WU Guo-hua LI Jian ZHU Hai-xia 

机构地区：[1]Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Public Health of Ministry of Agriculture,and Key Laboratory of Animal Virology of Ministry of Agriculture,Lanzhou 730046,China

出　　处：《Animal Husbandry and Feed Science》2010年第3期32-34,38,共4页动物与饲料科学(英文版)

基　　金：supported by the grants from the Special Fund for Agricultural Biotechnology of Gansu Province (092NKDA032);National Key Technology R&D Program (2006BAD06A11 and 2006BAD06A17)

摘　　要：To discriminate goatpoxvirus (GPV) and sheeppoxvirus (SPV), the p32 genes and G-protein-coupled chemokine receptor (GpCR) genes amplified from three SPV field isolates and two GPV field isolates were sequenced and compared with the corresponding sequences deposited in GenBank. Comparison of Hinf I restriction enzyme sites of p32 genes showed that there were two sites located at 391 and 691 bp, respectively, among all the SPV strains, and one site at 688 bp among GPV strains. Cladogram generated by sequence alignment of GpCR genes showed that the SPV and GPV belonged to two different branches. These results indicate that p32. and GpCR genes can be candidates used for discrimination of GPV and SPV.To discriminate goatpoxvirus (GPV) and sheeppoxvirus (SPV), the p32 genes and G-protein-coupled chemokine receptor (GpCR) genes amplified from three SPV field isolates and two GPV field isolates were sequenced and compared with the corresponding sequences deposited in GenBank. Comparison of Hinf I restriction enzyme sites of p32 genes showed that there were two sites located at 391 and 691 bp, respectively, among all the SPV strains, and one site at 688 bp among GPV strains. Cladogram generated by sequence alignment of GpCR genes showed that the SPV and GPV belonged to two different branches. These results indicate that p32. and GpCR genes can be candidates used for discrimination of GPV and SPV.

关 键 词：pG2 gene GpCRgene Goatpoxvirus Sheeppoxvirus DISCRIMINATION 

分 类 号：S852.65[农业科学—基础兽医学]